Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5 Pt 1
pubmed:dateCreated
1985-12-13
pubmed:abstractText
We have found that hypertonic medium inhibited the receptor-mediated uptake of the chemotactic peptide N-formylnorleucylleucylphenylalanine without affecting fluid-phase endocytosis by polymorphonuclear leukocytes (PMNs). Morphological and biochemical evidence demonstrated that cells in hypertonic medium did not accumulate peptide in a receptor-mediated manner. However, the cells continued to form endosomes containing fluid-phase markers. Furthermore, the content of these endosomes was processed normally, i.e., both digested and intact material were released into the medium. The inhibition of receptor-mediated uptake was a function of the tonicity. Partial inhibition occurred in 0.45 and 0.6 osmolar medium and maximal inhibition occurred in 0.75 osmolar medium. The inhibition was independent of the solute used to increase the tonicity: sodium chloride, sucrose, and lactose all inhibited uptake to similar extents. Hypertonic medium had little effect on saturable peptide binding. However, it did prevent the clustering of surface molecules as indicated by the inhibition of capping of fluorescent concanavalin A. In addition, hypertonic medium prevented the peptide-stimulated increase in cytosolic calcium levels as measured by quin 2 fluorescence. The tonicity dependence of the inhibition of quin 2 fluorescence paralleled the inhibition of receptor-mediated uptake.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/4055891-103882, http://linkedlifedata.com/resource/pubmed/commentcorrection/4055891-315944, http://linkedlifedata.com/resource/pubmed/commentcorrection/4055891-332066, http://linkedlifedata.com/resource/pubmed/commentcorrection/4055891-472759, http://linkedlifedata.com/resource/pubmed/commentcorrection/4055891-6147196, http://linkedlifedata.com/resource/pubmed/commentcorrection/4055891-6166391, http://linkedlifedata.com/resource/pubmed/commentcorrection/4055891-6304114, http://linkedlifedata.com/resource/pubmed/commentcorrection/4055891-6310757, http://linkedlifedata.com/resource/pubmed/commentcorrection/4055891-6715403, http://linkedlifedata.com/resource/pubmed/commentcorrection/4055891-7298722, http://linkedlifedata.com/resource/pubmed/commentcorrection/4055891-7375938, http://linkedlifedata.com/resource/pubmed/commentcorrection/4055891-7391138, http://linkedlifedata.com/resource/pubmed/commentcorrection/4055891-7463480
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0021-9525
pubmed:author
pubmed:issnType
Print
pubmed:volume
101
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1673-9
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1985
pubmed:articleTitle
Inhibition of receptor-mediated but not fluid-phase endocytosis in polymorphonuclear leukocytes.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.