Linked Life Data

4052458

Source:http://linkedlifedata.com/resource/pubmed/id/4052458

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1985-12-2
pubmed:abstractText
The release of 9-aminoacridine loaded into neutrophil granules has been monitored using quantitative fluorescence microscopy of individual rat neutrophils. Within the granule, the fluorescence of the dye was substantially quenched, but release into the surrounding medium restored fluorescence. From kinetic analysis of the increase in fluorescence it was shown that secretion from a single neutrophil in response to a low concentration of chemotactic peptide occurred in 'bursts'. Each 'burst' of secretion was of equal size and kinetics, which were equal to the size and kinetics of the smallest evoked response possible. A significant time-lag of 5-10 s between the arrival of the stimulus at the cell and the onset of secretion was recorded. It was therefore concluded that secretion from neutrophils was the result of release of quantal amounts of dye following a delay period.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0006-3002
pubmed:author
pubmed:issnType
Print
pubmed:day
30
pubmed:volume
847
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
15-9
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1985
pubmed:articleTitle
Quantal secretion and response lag demonstrated in single rat neutrophils.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't