Linked Life Data

4044522

Source:http://linkedlifedata.com/resource/pubmed/id/4044522

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1985-11-14
pubmed:abstractText
A method for isolating phototaxis-deficient (Pho-) mutants of Halobacterium halobium was developed. The procedure makes use of a flashing repellent light to induce frequent reversals of swimming direction by responsive cells, thereby impeding their migration along a small capillary and resulting in a spatial separation of the parent population and a population enriched for Pho- cells. Two classes of Pho- mutants were obtained by this selection scheme: those which have lost the chemotactic response (Che-) as well as phototaxis sensitivity (general taxis mutants), and those which are defective in steps specific to phototaxis (photosignaling mutants). In the latter class, several retinal synthesis mutants were isolated, as well as a strain which fit the expected properties of a mutant lacking a functional photoreceptor protein. On the basis of spectroscopic and swimming behavior studies, the retinal-containing protein, slow-cycling or sensory rhodopsin (SR), was previously proposed to be a dual-function sensory receptor mediating both attractant and repellent photosensing. The receptor mutant Pho81 fulfills two predictions which provide direct genetic evidence for this proposal. The mutant has lost SR photoactivity as determined by spectroscopic measurements, and it has simultaneously lost both attractant and repellent phototaxis sensitivity. Comparison of [3H]retinal-labeled membrane proteins from the mutant and its SR-containing parent implicated a 25,000 Mr polypeptide as the chromophoric polypeptide of SR.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/4044522-13534434, http://linkedlifedata.com/resource/pubmed/commentcorrection/4044522-3881422, http://linkedlifedata.com/resource/pubmed/commentcorrection/4044522-4560688, http://linkedlifedata.com/resource/pubmed/commentcorrection/4044522-4957395, http://linkedlifedata.com/resource/pubmed/commentcorrection/4044522-6289299, http://linkedlifedata.com/resource/pubmed/commentcorrection/4044522-6502700, http://linkedlifedata.com/resource/pubmed/commentcorrection/4044522-6504161, http://linkedlifedata.com/resource/pubmed/commentcorrection/4044522-6522400, http://linkedlifedata.com/resource/pubmed/commentcorrection/4044522-6616008, http://linkedlifedata.com/resource/pubmed/commentcorrection/4044522-6748081, http://linkedlifedata.com/resource/pubmed/commentcorrection/4044522-6838616, http://linkedlifedata.com/resource/pubmed/commentcorrection/4044522-6959114, http://linkedlifedata.com/resource/pubmed/commentcorrection/4044522-857951
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0021-9193
pubmed:author
pubmed:issnType
Print
pubmed:volume
164
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
282-7
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1985
pubmed:articleTitle
Selection and properties of phototaxis-deficient mutants of Halobacterium halobium.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't