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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1977-6-11
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pubmed:abstractText |
A comparative study of several published methods for sheep red blood cell-T-lymphocyte rosette formation was performed. Maximum SRBC-rosette formation occurred with AET treated SRBC in medium supplemented with 20% FCS or with untreated SRBC in 100% FCS. Prolongation of the 4 degrees C incubation period from 4 to 18 h enchanced rosette formation. Fluorescein diacetate staining significantly increased calculated percentage of rosette formation. Fluorescein diacetate staining significantly increased calculated percentage of rosette-forming lyphocytes by allowing accurate indentification of the central lymphocytes in morulas.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
0022-1759
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pubmed:author |
|
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pubmed:issnType |
Print
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pubmed:volume |
15
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
239-45
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:404361-Animals,
pubmed-meshheading:404361-Culture Media,
pubmed-meshheading:404361-Erythrocytes,
pubmed-meshheading:404361-Fluoresceins,
pubmed-meshheading:404361-Glutaral,
pubmed-meshheading:404361-Humans,
pubmed-meshheading:404361-Immunologic Techniques,
pubmed-meshheading:404361-Neuraminidase,
pubmed-meshheading:404361-Sheep,
pubmed-meshheading:404361-T-Lymphocytes,
pubmed-meshheading:404361-Time Factors,
pubmed-meshheading:404361-Trypsin,
pubmed-meshheading:404361-beta-Aminoethyl Isothiourea
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pubmed:year |
1977
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pubmed:articleTitle |
A comparative study of procedures for sheep erythrocyte-human-T-lymphocyte rosette formation.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.
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