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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
|
pubmed:dateCreated |
1985-9-3
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pubmed:abstractText |
To investigate the intracellular molecular events during leukemic cell proliferation, we have examined the method of ghost-mediated microinjection of macromolecules into leukemic cell line cells (HL-60). Samples were packed into red cell ghosts. Microinjection was performed by the fusion of ghosts and HL-60 cells using the hemagglutinating virus of Japan (HVJ). Fusion rate was about 80-90%, when determined by the injection of FITC-labeled globulins (IgG) or diphtheria toxin fragment A into HL-60 cells. When the nuclear protein extract from normal granulocytes was injected into HL-60 cells, their growth was significantly suppressed. The injection of the nuclear protein extract from HL-60 itself into HL-60 cells did not inhibit their growth. This finding suggests that leukemic cells may be deficient in intracellular regulatory factors which have suppressive activity on cell growth.
|
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical | |
pubmed:status |
MEDLINE
|
pubmed:month |
Jul
|
pubmed:issn |
0006-291X
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pubmed:author | |
pubmed:issnType |
Print
|
pubmed:day |
16
|
pubmed:volume |
130
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
257-63
|
pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:4040753-Animals,
pubmed-meshheading:4040753-Cell Fusion,
pubmed-meshheading:4040753-Erythrocyte Membrane,
pubmed-meshheading:4040753-Growth Inhibitors,
pubmed-meshheading:4040753-Humans,
pubmed-meshheading:4040753-Immunoglobulin G,
pubmed-meshheading:4040753-Leukemia, Experimental,
pubmed-meshheading:4040753-Microinjections,
pubmed-meshheading:4040753-Nucleoproteins
|
pubmed:year |
1985
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pubmed:articleTitle |
Microinjection of macromolecules into leukemic cells by cell fusion technique: search for intracellular growth-suppressive factors.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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