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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
1985-5-23
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pubmed:abstractText |
Uterine plasma membrane preparations were obtained by centrifugation on discontinuous sucrose gradients. The specific activity of the plasma membrane marker 5'-nucleotidase was increased 10-fold while the specific activity of glucose-6-phosphatase was increased 3-fold. Electron microscopy showed mainly closed vesicles having diameters mainly in the range of 0.1 to 0.4 micron and an absence of other recognizable organelles such as mitochondria. D-Glucose transport was inhibited by sulfhydryl reagents, phloretin, and cytochalasin B. Uptake was prevented at high osmotic pressures. The Km of glucose transport was 12.2 +/- 1.1 mM. Studies of the inhibition of [3H]cytochalasin B binding by D-glucose indicated that the value of the Kd of the cytochalasin B-transporter complex was larger than 1 microM. These data demonstrate the potential usefulness of these preparations in the study of glucose transport in rat uterus and its control by steroid hormones.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0006-3002
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
11
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pubmed:volume |
814
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
341-6
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pubmed:dateRevised |
2003-11-14
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pubmed:meshHeading |
pubmed-meshheading:4038886-Animals,
pubmed-meshheading:4038886-Biological Transport, Active,
pubmed-meshheading:4038886-Carrier Proteins,
pubmed-meshheading:4038886-Cell Membrane,
pubmed-meshheading:4038886-Centrifugation, Density Gradient,
pubmed-meshheading:4038886-Cytochalasin B,
pubmed-meshheading:4038886-Female,
pubmed-meshheading:4038886-Kinetics,
pubmed-meshheading:4038886-Microscopy, Electron,
pubmed-meshheading:4038886-Monosaccharide Transport Proteins,
pubmed-meshheading:4038886-Phloretin,
pubmed-meshheading:4038886-Rats,
pubmed-meshheading:4038886-Rats, Inbred Strains,
pubmed-meshheading:4038886-Uterus
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pubmed:year |
1985
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pubmed:articleTitle |
Glucose transport by uterine plasma membranes.
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pubmed:publicationType |
Journal Article
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