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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
15
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pubmed:dateCreated |
1985-10-8
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pubmed:abstractText |
Analogs of alternating purine-pyrimidine DNA polymers such as poly(dA-dT)-poly(dA-dT) can be made with phosphorothioate groups in the DNA backbone. A phosphorothioate diester at the 5'-purine-pyrimidine-3' step causes a significant lowering of the polymer's melting temperature compared to a phosphorothioate diester at the 5'-pyrimidine-purine-3' step. This may occur because sulfur substitution increases anionic charge density in the DNA minor groove and 5'-purine-pyrimidine-3' steps narrow the minor groove. The ability to modulate charge density in the DNA backbone via sulfur substitution should prove useful in studies of sequence-dependent conformational changes in DNA.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/4034395-1067602,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4034395-284337,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4034395-4589546,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4034395-468810,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4034395-4878044,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4034395-513130,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4034395-5444162,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4034395-5760542,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4034395-588563,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4034395-6273590,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4034395-6327070,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4034395-6345056,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4034395-6354262,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4034395-6400829,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4034395-6572358,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4034395-6574849,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4034395-6712923,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4034395-6854636,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4034395-6854642,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4034395-7020579,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4034395-7057893,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4034395-7154084,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4034395-7174662,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4034395-7285918,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4034395-7318827
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0305-1048
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
12
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pubmed:volume |
13
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
5707-16
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:4034395-Crystallization,
pubmed-meshheading:4034395-DNA,
pubmed-meshheading:4034395-Hot Temperature,
pubmed-meshheading:4034395-Magnetic Resonance Spectroscopy,
pubmed-meshheading:4034395-Nucleic Acid Conformation,
pubmed-meshheading:4034395-Nucleic Acid Heteroduplexes,
pubmed-meshheading:4034395-Organothiophosphorus Compounds,
pubmed-meshheading:4034395-Poly dA-dT,
pubmed-meshheading:4034395-Pyrrolidines,
pubmed-meshheading:4034395-Solutions,
pubmed-meshheading:4034395-Structure-Activity Relationship,
pubmed-meshheading:4034395-Thermodynamics,
pubmed-meshheading:4034395-Thiophosphoric Acid Esters
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pubmed:year |
1985
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pubmed:articleTitle |
Evidence for sequence-specific conformational changes in DNA from the melting temperatures of DNA phosphorothioate derivatives.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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