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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
1985-9-30
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pubmed:abstractText |
Growth of embryonic chicken sternal chondrocytes in the presence of phorbol-12-myristate-13-acetate (PMA), a potent tumor promoter, resulted in a dramatic morphological change from spherical floating cells to adherent fibroblastic cells. This morphological change was accompanied by a quantitative switch from synthesis of cartilage-specific type II procollagen to type I procollagen. Type II procollagen mRNA levels decreased 10-fold in PMA-treated cells. Activation of type I collagen genes led to the accumulation of type I procollagen mRNA levels comparable to those of type II mRNA in these cells. However, only type I procollagen mRNA was translated. In addition to gene activation, unprocessed pro alpha 1(I) transcripts present at low levels in control chondrocytes were processed to mature mRNA species. Redifferentiation of PMA-treated chondrocytes was possible if cells were removed from PMA after the morphological change and cessation of type II procollagen synthesis but before detectable amounts of type I procollagen were synthesized. Production of type I collagen thus marks a late phase of chondrocyte "dedifferentiation" from which reversion is no longer possible. Redifferentiated cell populations contained 24-fold more pro alpha 1(II) collagen mRNA than pro alpha 1(I) collagen mRNA, but the rates of procollagen synthesis were comparable. This suggests that the PMA-mediated dedifferentiation of chondrocytes as well as their redifferentiation is under both transcriptional and posttranscriptional regulation.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/4033659-1060132,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4033659-1064040,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4033659-1175627,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4033659-16590779,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4033659-196766,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4033659-207312,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4033659-221453,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/4033659-364479,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/4033659-526264,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4033659-5271750,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/4033659-976346
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0270-7306
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
5
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1415-24
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:4033659-Animals,
pubmed-meshheading:4033659-Cartilage,
pubmed-meshheading:4033659-Cell Differentiation,
pubmed-meshheading:4033659-Cell Division,
pubmed-meshheading:4033659-Chick Embryo,
pubmed-meshheading:4033659-Collagen,
pubmed-meshheading:4033659-Gene Expression Regulation,
pubmed-meshheading:4033659-Phorbols,
pubmed-meshheading:4033659-Procollagen,
pubmed-meshheading:4033659-RNA, Messenger,
pubmed-meshheading:4033659-Tetradecanoylphorbol Acetate,
pubmed-meshheading:4033659-Transcriptional Activation
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pubmed:year |
1985
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pubmed:articleTitle |
Collagen expression in embryonic chicken chondrocytes treated with phorbol myristate acetate.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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