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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3
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pubmed:dateCreated |
1985-10-7
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pubmed:abstractText |
Two molecular defects involving the spectrin heterodimer (SpD) contact site of the alpha chain (the alpha I domain) were previously identified using limited tryptic digestion followed by two-dimensional isoelectric focusing/sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Both are characterized by atypical peptide maps which reveal a marked decrease of the 80,000-dalton alpha I domain and a formation of new major peptides of either 74,000 (Sp alpha I/74) or 46,000 (Sp alpha I/46) daltons. We now report a third variant of the spectrin alpha chain, designated Sp alpha I/65, in three unrelated black families. In all three probands, the percentage of SpD in the low ionic strength (O degrees C) membrane extracts was increased to 19% to 32%. One- and two-dimensional electrophoretic separations of limited tryptic digests of spectrin from all three probands revealed a decrease of the alpha I domain of spectrin and the concomitant appearance of peptides at 65,000 daltons and isoelectric points ranging from 5.2 to 5.3. The abnormal 65,000-dalton peptides could be stained with an antiserum which had been raised against the alpha I domain, indicating that it was derived from the alpha I domain.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
AIM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Sep
|
pubmed:issn |
0006-4971
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
66
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
706-9
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:4027386-Adult,
pubmed-meshheading:4027386-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:4027386-Elliptocytosis, Hereditary,
pubmed-meshheading:4027386-Female,
pubmed-meshheading:4027386-Genetic Variation,
pubmed-meshheading:4027386-Humans,
pubmed-meshheading:4027386-Infant,
pubmed-meshheading:4027386-Isoelectric Focusing,
pubmed-meshheading:4027386-Macromolecular Substances,
pubmed-meshheading:4027386-Middle Aged,
pubmed-meshheading:4027386-Peptides,
pubmed-meshheading:4027386-Sodium Dodecyl Sulfate,
pubmed-meshheading:4027386-Spectrin
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pubmed:year |
1985
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pubmed:articleTitle |
Sp alpha I/65: a new variant of the alpha subunit of spectrin in hereditary elliptocytosis.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Case Reports,
Research Support, Non-U.S. Gov't
|