Linked Life Data

4019471

Source:http://linkedlifedata.com/resource/pubmed/id/4019471

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
16
pubmed:dateCreated
1985-8-29
pubmed:abstractText
Marrow cells induced toward erythroid differentiation by treatment with erythropoietin respond by increasing the rates of iron uptake and hemoglobin synthesis. Study of the enzymes of heme biosynthesis during erythroid differentiation suggests that induction of heme synthesis in these cells is regulated by synthesis of porphobilinogen deaminase. The activities of delta-aminolevulinic acid synthase, gamma, delta-dioxovaleric acid transaminase, delta-aminolevulinic acid dehydratase, and ferrochelatase were not affected significantly by treatment of suppressed marrow cells with erythropoietin over a period of 4 days, whereas that of porphobilinogen deaminase was increased by as much as 3.5-fold by the 3rd day of incubation. The time course of increase in porphobilinogen deaminase activity was parallel to that of the increase in heme synthesis. Moreover, when porphobilinogen deaminase activity was compared in marrow cells exposed to increased levels of erythropoietin in vivo (hyperplastic marrow) and marrow cells exposed to lowered levels of erythropoietin in vivo (suppressed marrow), the activity in the former case was greater than that in normal cells and for the latter type of cell it was lower than normal. Experiments using actinomycin D and cycloheximide suggest that transcription is required for the erythropoietin-induced porphobilinogen deaminase activity, indicating that induction is probably at the level of de novo synthesis of enzyme.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
5
pubmed:volume
260
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
9251-7
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1985
pubmed:articleTitle
The regulation of heme biosynthesis during erythropoietin-induced erythroid differentiation.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S.