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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3
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pubmed:dateCreated |
1985-9-4
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pubmed:abstractText |
The cell-free extract of a cytochrome P-450-producing fungus, Fusarium oxysporum, was found to catalyze the hydroxylation of fatty acids. Three product isomers were formed from a single fatty acid. The products from lauric acid were identified by mass-spectrometry as 9-, 10-, and 11-hydroxydodecanoic acids, and those from palmitic acid as 13-, 14-, and 15-hydroxyhexadecanoic acids. The ratio of the isomers formed was 50 : 36 : 14 in the case of laurate hydroxylation, and 37 : 47 : 16 in the case of palmitate. The reaction was dependent on both NADPH (or NADH) and molecular oxygen,and was strongly inhibited by carbon monoxide, menadione, or the antibody to purified Fusarium P-450. Further, lauric acid induced a type I spectral change in purified Fusarium P-450. Further, lauric acid induced a type I spectral change in purified Fusarium P-450 with an apparent Kd of 0.3 mM. The hydroxylase activity together with cytochrome P-450 could be detected in both the soluble and microsome fractions, and the activity was almost proportional to the amount of cytochrome P-450 reducible with NADPH. It can be concluded from these results that Fusarium P-450 reducible with NADPH. It can be concluded from these results that Fusarium P-450 is involved in the (omega-1)-, (omega-2)-, and (omega-3)-hydroxylation of fatty acids catalyzed by the cell-free extract of the fungus.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies,
http://linkedlifedata.com/resource/pubmed/chemical/Cytochrome P-450 Enzyme System,
http://linkedlifedata.com/resource/pubmed/chemical/Fatty Acids,
http://linkedlifedata.com/resource/pubmed/chemical/Lauric Acids,
http://linkedlifedata.com/resource/pubmed/chemical/Mixed Function Oxygenases,
http://linkedlifedata.com/resource/pubmed/chemical/NADP,
http://linkedlifedata.com/resource/pubmed/chemical/lauric acid
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0021-924X
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
97
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
755-63
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pubmed:dateRevised |
2007-12-19
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pubmed:meshHeading |
pubmed-meshheading:4019433-Antibodies,
pubmed-meshheading:4019433-Catalysis,
pubmed-meshheading:4019433-Chromatography, High Pressure Liquid,
pubmed-meshheading:4019433-Cytochrome P-450 Enzyme System,
pubmed-meshheading:4019433-Fatty Acids,
pubmed-meshheading:4019433-Fusarium,
pubmed-meshheading:4019433-Hydroxylation,
pubmed-meshheading:4019433-Lauric Acids,
pubmed-meshheading:4019433-Mass Spectrometry,
pubmed-meshheading:4019433-Microsomes,
pubmed-meshheading:4019433-Mixed Function Oxygenases,
pubmed-meshheading:4019433-NADP,
pubmed-meshheading:4019433-Oxidation-Reduction
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pubmed:year |
1985
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pubmed:articleTitle |
Subterminal hydroxylation of fatty acids by a cytochrome P-450-dependent enzyme system from a fungus, Fusarium oxysporum.
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pubmed:publicationType |
Journal Article
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