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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1977-3-21
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pubmed:abstractText |
A procedure is described for the selective isolation of temperature-sensitive replication-deficient mutants of Bacillus subtilis phage SPO1. A modification of the procedure permits the isolation of temperature-sensitive mutants in specific cistrons of interest. The applicability of these procedures to other viral systems is discussed. The mutations isolated were assigned to eight replication-deficient cistrons, with the cold-sensitive mutations showing a distribution strikingly different from that of the heat-sensitive mutations. As a preliminary to the identification of initiation-deficient mutants, the mutants were divided into three classes on the basis of their ability to synthesize DNA after a shift to nonpermissive temperature. We also report two incidental results: (i) the SPO1 dUMP hydroxymethylase, like the T4 dCMP hydroxymethylase, may be part of a multifunctional complex; and (ii) mutants were isolated that were replication positive but lysis deficient and failed to complement one of the replication-deficient mutants.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/401897-1062786,
http://linkedlifedata.com/resource/pubmed/commentcorrection/401897-14156925,
http://linkedlifedata.com/resource/pubmed/commentcorrection/401897-4200923,
http://linkedlifedata.com/resource/pubmed/commentcorrection/401897-4212248,
http://linkedlifedata.com/resource/pubmed/commentcorrection/401897-4276314,
http://linkedlifedata.com/resource/pubmed/commentcorrection/401897-4579012,
http://linkedlifedata.com/resource/pubmed/commentcorrection/401897-4583886,
http://linkedlifedata.com/resource/pubmed/commentcorrection/401897-4612038,
http://linkedlifedata.com/resource/pubmed/commentcorrection/401897-4622590,
http://linkedlifedata.com/resource/pubmed/commentcorrection/401897-4623500,
http://linkedlifedata.com/resource/pubmed/commentcorrection/401897-4623501,
http://linkedlifedata.com/resource/pubmed/commentcorrection/401897-4628252,
http://linkedlifedata.com/resource/pubmed/commentcorrection/401897-4628367,
http://linkedlifedata.com/resource/pubmed/commentcorrection/401897-4629687,
http://linkedlifedata.com/resource/pubmed/commentcorrection/401897-4885498,
http://linkedlifedata.com/resource/pubmed/commentcorrection/401897-4887510,
http://linkedlifedata.com/resource/pubmed/commentcorrection/401897-4895536,
http://linkedlifedata.com/resource/pubmed/commentcorrection/401897-4959194,
http://linkedlifedata.com/resource/pubmed/commentcorrection/401897-4995759,
http://linkedlifedata.com/resource/pubmed/commentcorrection/401897-4996233,
http://linkedlifedata.com/resource/pubmed/commentcorrection/401897-4999125,
http://linkedlifedata.com/resource/pubmed/commentcorrection/401897-5002938,
http://linkedlifedata.com/resource/pubmed/commentcorrection/401897-5167656,
http://linkedlifedata.com/resource/pubmed/commentcorrection/401897-5556293,
http://linkedlifedata.com/resource/pubmed/commentcorrection/401897-5663335,
http://linkedlifedata.com/resource/pubmed/commentcorrection/401897-806080,
http://linkedlifedata.com/resource/pubmed/commentcorrection/401897-809034,
http://linkedlifedata.com/resource/pubmed/commentcorrection/401897-810655,
http://linkedlifedata.com/resource/pubmed/commentcorrection/401897-815553
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0022-538X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
21
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
54-60
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:401897-Bacillus subtilis,
pubmed-meshheading:401897-Bacteriophages,
pubmed-meshheading:401897-Cold Temperature,
pubmed-meshheading:401897-DNA, Viral,
pubmed-meshheading:401897-DNA Replication,
pubmed-meshheading:401897-Genes,
pubmed-meshheading:401897-Hot Temperature,
pubmed-meshheading:401897-Methyltransferases,
pubmed-meshheading:401897-Mutation,
pubmed-meshheading:401897-Transferases
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pubmed:year |
1977
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pubmed:articleTitle |
Selective screening procedure for the isolation of heat- and cold-sensitive, DNA replication-deficient mutants of bacteriophage SPO1 and preliminary characterization of the mutants isolated.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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