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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
1985-8-28
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pubmed:abstractText |
We have developed a new methodology to examine effector-cell-mediated immune attack using liposomes as targets. Hydrogen peroxide-associated killing of liposomes was observed with fluorescence intensification microscopy. Liposomes were composed of 98-99 mol % egg phosphatidylcholine and 1-2 mol % dinitrophenyl lipid hapten. Anti-dinitrophenyl IgG antibody was used to opsonize liposomes. Liposomes were loaded with dihydroxymandelic acid (DHMA) and peroxidase. Macrophage- or neutrophil-mediated recognition of liposomes triggers the release of H2O2 and other oxidative products. Upon interaction of H2O2 or OH radical with liposome contents, DHMA dimerizes forming a fluorescent derivative. Our studies indicate that individual living neutrophils and macrophages deposit oxidative products in a heterogenous fashion among bound targets.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/4016201-171281,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4016201-218577,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4016201-220614,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4016201-279008,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4016201-279285,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4016201-310125,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4016201-315069,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4016201-4365759,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4016201-5634544,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4016201-5743279,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4016201-6176223,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4016201-6250449,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4016201-6493224,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4016201-6688737,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4016201-6800035,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4016201-6893805,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4016201-6935669,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4016201-6941268,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4016201-6967736,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4016201-7061587,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4016201-71015,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4016201-7197629,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4016201-7251651,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4016201-7430632,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4016201-7448176,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4016201-748181,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4016201-77736
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0006-3495
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
47
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
837-40
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:4016201-Antibodies,
pubmed-meshheading:4016201-Humans,
pubmed-meshheading:4016201-Hydrogen Peroxide,
pubmed-meshheading:4016201-Immunity, Cellular,
pubmed-meshheading:4016201-Liposomes,
pubmed-meshheading:4016201-Macrophages,
pubmed-meshheading:4016201-Microscopy, Fluorescence,
pubmed-meshheading:4016201-Neutrophils,
pubmed-meshheading:4016201-Phagocytes,
pubmed-meshheading:4016201-Phagocytosis,
pubmed-meshheading:4016201-Phosphatidylcholines
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pubmed:year |
1985
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pubmed:articleTitle |
Novel fluorescence method to visualize antibody-dependent hydrogen peroxide-associated "killing" of liposomes by phagocytes.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.
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