Linked Life Data

4008569

Source:http://linkedlifedata.com/resource/pubmed/id/4008569

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1985-8-21
pubmed:abstractText
A rapid, accurate and sensitive method for the determination of free hydroxyproline and proline in plasma and of total hydroxyproline in urine has been developed. Free imino acids and internal standard are extracted from plasma by trichloroacetic acid precipitation of protein and they are selectively derivatized with 4-chloro-7-nitrobenzofurazan, after reaction of the acid extract with o-phthalaldehyde. The highly fluorescent adducts of imino acids are separated on a Spherisorb ODS 2 reversed-phase column using acetonitrile-0.1 M sodium phosphate buffer, pH 7.2 (9:91, v/v) as mobile phase, followed by fluorometric detection. Total hydroxyproline determination in urine hydrolysates is carried out by reaction of the imino acid with 4-chloro-7-nitrobenzofurazan after clean-up on a Sep-Pak C18 cartridge of the o-phthalaldehyde-treated sample, high-performance liquid chromatographic separation and fluorometric quantitation of the derivative.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0021-9673
pubmed:author
pubmed:issnType
Print
pubmed:day
3
pubmed:volume
339
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
285-92
pubmed:dateRevised
2004-11-17
pubmed:meshHeading
pubmed:year
1985
pubmed:articleTitle
High-performance liquid chromatographic analysis of free hydroxyproline and proline in blood plasma and of free and peptide-bound hydroxyproline in urine.
pubmed:publicationType
Journal Article