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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1985-8-1
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pubmed:abstractText |
Reversed-phase high-performance liquid chromatography was used to purify an inhibitory glycopeptide where resolution and recovery were enhanced by using urea or guanidine-HCl-isopropanol-water as a solvent system. Isopropanol alone or other solvent systems that have been proposed for such purification steps were not effective in eluting hydrophobic proteins from the reversed-phase column. The application of the urea or guanidine-HCl solvent systems in the separation and purification of membrane proteins, and other hydrophobic macromolecules, could greatly enhance recovery and efficiency of purification.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0021-9673
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
17
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pubmed:volume |
324
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
173-80
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:4008552-1-Propanol,
pubmed-meshheading:4008552-Animals,
pubmed-meshheading:4008552-Cattle,
pubmed-meshheading:4008552-Cells, Cultured,
pubmed-meshheading:4008552-Chromatography, High Pressure Liquid,
pubmed-meshheading:4008552-Drug Stability,
pubmed-meshheading:4008552-Growth Inhibitors,
pubmed-meshheading:4008552-Guanidine,
pubmed-meshheading:4008552-Guanidines,
pubmed-meshheading:4008552-Membrane Proteins,
pubmed-meshheading:4008552-Mice,
pubmed-meshheading:4008552-Mice, Inbred BALB C,
pubmed-meshheading:4008552-Solvents,
pubmed-meshheading:4008552-Urea
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pubmed:year |
1985
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pubmed:articleTitle |
Use of a urea and guanidine-HCl-propanol solvent system to purify a growth inhibitory glycopeptide by high-performance liquid chromatography.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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