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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1985-8-6
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pubmed:abstractText |
A malignant cell line (clone S1) isolated after co-transfection of normal NIH3T3 DNA and Moloney leukemia virus long terminal repeat (Mo-LTR) sequences has previously been described to contain an activated c-raf oncogene. Here, we report the isolation by molecular cloning and the structural analysis of the LTR-activated c-raf gene. As shown by Southern blot and nucleotide sequence analyses, the transfected Mo-LTR sequences integrated into the 5th intron of the endogenous c-raf proto-oncogene. This intragenic LTR insertion led to the expression of high levels of LTR-U5-c-raf hybrid transcripts indicating an initiation of transcription from the Mo-LTR promoter. Transcriptional activation of c-raf is accompanied by the synthesis of large amounts of cytoplasmic c-raf protein. Immunoblot analysis suggests that the proteins encoded by the LTR-activated c-raf gene are truncated compared with the normal c-raf gene product(s). Our results indicate a promoter insertion mechanism of c-raf activation.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/4006904-322279,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4006904-388439,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4006904-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4006904-6246368,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4006904-6251455,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4006904-6257399,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4006904-6276760,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4006904-6276775,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4006904-6300781,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4006904-6307525,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4006904-6308607,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4006904-6315951,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4006904-6323249,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4006904-6325930,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4006904-6328272,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4006904-6329737,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4006904-6541297,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4006904-6946480,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4006904-7066985,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4006904-7360280
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0261-4189
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
4
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
693-8
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:4006904-Animals,
pubmed-meshheading:4006904-Base Sequence,
pubmed-meshheading:4006904-Cell Transformation, Neoplastic,
pubmed-meshheading:4006904-Cells, Cultured,
pubmed-meshheading:4006904-Cloning, Molecular,
pubmed-meshheading:4006904-DNA, Viral,
pubmed-meshheading:4006904-Gene Amplification,
pubmed-meshheading:4006904-Gene Expression Regulation,
pubmed-meshheading:4006904-Genetic Linkage,
pubmed-meshheading:4006904-Mice,
pubmed-meshheading:4006904-Moloney murine leukemia virus,
pubmed-meshheading:4006904-Oncogenes,
pubmed-meshheading:4006904-Promoter Regions, Genetic,
pubmed-meshheading:4006904-Repetitive Sequences, Nucleic Acid,
pubmed-meshheading:4006904-Transcription, Genetic
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pubmed:year |
1985
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pubmed:articleTitle |
Integration of transfected LTR sequences into the c-raf proto-oncogene: activation by promoter insertion.
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pubmed:publicationType |
Journal Article
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