Linked Life Data

4004841

Source:http://linkedlifedata.com/resource/pubmed/id/4004841

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1985-7-24
pubmed:abstractText
Ejaculated bull spermatozoa separated from seminal plasma by repeated washing were used to study the effects of various substrates on respiration, mitochondrial energization, cellular ATP/ADP ratios, and motility. The respiratory rate in the presence of lactate, pyruvate, acetate or glycerol was about two to three times higher compared to cells oxidizing amino acids, fatty acids, or endogenous substrates. When well oxidizable substrates were present, the uncoupler FCCP produced a marked increase in respiration (two to three times at optimal FCCP concentrations). Mitochondrial energization was monitored in intact spermatozoa by spectrophotometry with the dye safranin. Lactate and pyruvate yielded more pronounced mitochondrial energization than did alanine or endogenous substrates. Cellular ATP/ADP ratios, with lactate, pyruvate, acetate or glycerol, were found to range from 6 to 10, whereas endogenous substrates and amino acids were able to establish ATP/ADP quotients as low as about 1 only. The capacity to oxidize various substrates was clearly reflected by the amount of motile cells. Motility in the presence of lactate, acetate or glycerol was seen in more than 60% of spermatozoa, while spermatozoa in the presence of alanine or butyrate showed a motility not above the endogenous level (approx. 30% motile cells). The experiments demonstrated that cellular performance is highly dependent on the energy state as made possible by the capacity of substrate utilization.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0232-766X
pubmed:author
pubmed:issnType
Print
pubmed:volume
44
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
411-20
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1985
pubmed:articleTitle
Effect of various substrates on mitochondrial and cellular energy state of intact spermatozoa.
pubmed:publicationType
Journal Article, Comparative Study, In Vitro