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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
1985-7-25
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pubmed:abstractText |
In this paper, an alternative procedure for the histochemical detection of HRP using amonium heptamolybdate (AHM) as a stabilizing agent and tetramethyl benzidine (TMB) as a chromogen is reported. This procedure avoids the two main problems that occur in previous methods using sodium nitroferricyanide (SNF) as the stabilizer, namely, the appearance of needle-shaped crystals at non-specific anatomical sites, and intensive tissue shrinkage. A comparative study of both, the TMB-AHM and TMB-SNF methods, was performed in the analysis of cerebral cortex afferent connections of the lizard Podarcis hispanica. This study demonstrates that the two methods are of similar sensitivity. The TMB-AHM reaction can be carried out at physiological pH (from 6 to 8), thus, avoiding tissue contraction. The reaction product is of an intense blue-green colour and, as with the TMB-SNF method, shows granulation. The appearance of non-specific precipitates is completely avoided when the incubation medium is maintained at a pH in excess of 5.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/3,3',5,5'-tetramethylbenzidine,
http://linkedlifedata.com/resource/pubmed/chemical/Benzidines,
http://linkedlifedata.com/resource/pubmed/chemical/Chromogenic Compounds,
http://linkedlifedata.com/resource/pubmed/chemical/Horseradish Peroxidase,
http://linkedlifedata.com/resource/pubmed/chemical/Peroxidases
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0165-0270
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
13
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
131-8
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:3999803-Animals,
pubmed-meshheading:3999803-Benzidines,
pubmed-meshheading:3999803-Brain,
pubmed-meshheading:3999803-Cerebral Cortex,
pubmed-meshheading:3999803-Chromogenic Compounds,
pubmed-meshheading:3999803-Histocytochemistry,
pubmed-meshheading:3999803-Horseradish Peroxidase,
pubmed-meshheading:3999803-Lizards,
pubmed-meshheading:3999803-Neural Pathways,
pubmed-meshheading:3999803-Neuroanatomy,
pubmed-meshheading:3999803-Peroxidases,
pubmed-meshheading:3999803-Raphe Nuclei,
pubmed-meshheading:3999803-Septum Pellucidum,
pubmed-meshheading:3999803-Thalamic Nuclei
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pubmed:year |
1985
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pubmed:articleTitle |
A new stabilizing agent for the tetramethyl benzidine (TMB) reaction product in the histochemical detection of horseradish peroxidase (HRP).
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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