Linked Life Data

3996410

Source:http://linkedlifedata.com/resource/pubmed/id/3996410

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1985-7-17
pubmed:abstractText
Structural studies were carried out on the polymer chains and their linkage regions in two kinds of teichoic acids, poly(N-acetylglucosamine 1-phosphate) [poly(GlcNAc-1-P)] and glycerol teichoic acid, bound to peptidoglycan in the cell walls of Bacillus pumilus AHU 1650. The poly(GlcNAc-1-P)-glycan complex isolated from lysozyme digests of the cell walls contained mannosamine and glycerol as minor components. On the basis of proton NMR spectroscopic data and isolation of N-acetylglucosamine 4-phosphate from acid hydrolysates, the poly(GlcNAc-1-P) was shown to be a polymer in which N-acetylglucosamine 1-phosphate units are joined at C-4 of the glucosamine residues. Mild alkaline hydrolysis of the poly(GlcNAc-1-P)-glycan complex gave a mannosamine-linked glycan fragment and the acidic polymer fraction that contained glycerol residues. Mild acid treatment of the mannosamine-linked glycan fragment gave the linkage disaccharide, ManNAc(beta 1----4)GlcNAc, whereas the acidic polymer fraction was degraded by this treatment into N-acetylglucosamine 4-phosphate and a glycerol-containing fragment characterized as P-(Gro-P)7 (Gro = glycerol). On the other hand, direct mild acid hydrolysis of the complex gave a fragment characterized as P-(Gro-P)7-ManNAc(beta 1----4)GlcNAc. These results lead to a conclusion that in the cell walls the poly(GlcNAc-1-P) chain is attached to peptidoglycan through a linkage unit, (Gro-P)7-ManNAc(beta 1----4)GlcNAc. By means of similar procedures, it was shown that the other cell wall polymer, glycerol teichoic acid, is also attached to peptidoglycan through the same disaccharide, ManNAc(beta 1----4)GlcNAc.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0014-2956
pubmed:author
pubmed:issnType
Print
pubmed:day
3
pubmed:volume
149
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
331-6
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
1985
pubmed:articleTitle
Structural studies on the linkage unit between poly(N-acetylglucosamine 1-phosphate) and peptidoglycan in cell walls of Bacillus pumilus AHU 1650.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't