Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1985-5-24
pubmed:abstractText
From the X-ray co-ordinates of bovine trypsin and its complexes with substrate analogues (benzamidine) and with soya-bean trypsin inhibitor, a peptide (TP) was designed and synthesized by surface-simulation synthesis, a concept previously introduced by this laboratory, to mimic the binding site of trypsin. Also, a control peptide (CTP) was synthesized that contained all the amino acids present in the TP peptide, except that their order was randomized. The radioiodinated TP peptide bound specifically to adsorbents of benzamidine, whereas the control CTP peptide exhibited no binding activity. Conjugates to succinyl (3-carboxypropionyl)-lysozyme of the TP peptide, control CTP peptide and other unrelated peptides were examined by a radiometric binding assay for the ability to bind soya-bean trypsin inhibitor and human alpha 1-antitrypsin. Conjugates of the TP peptide exhibited considerable binding activity to adsorbents of soya-bean trypsin inhibitor or alpha 1-antitrypsin. None of the other peptide conjugates possessed any binding activity. Action of the active-site-directed reagents phenylmethanesulphonyl fluoride and di-isopropyl phosphorofluoridate on free TP and CTP peptides resulted in the modification of a serine residue in the TP peptide whereas the CTP peptide remained unaltered. The TP peptide, either in the free form or as a conjugate on succinyl-lysozyme, had no enzymic activity on protein substrates or on tosylarginine methyl ester. These findings indicated that the binding activity of an enzyme was well mimicked by the surface-stimulation peptide but that reproduction of the catalytic activity was not obtained.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/3994669-1086102, http://linkedlifedata.com/resource/pubmed/commentcorrection/3994669-14450081, http://linkedlifedata.com/resource/pubmed/commentcorrection/3994669-4306345, http://linkedlifedata.com/resource/pubmed/commentcorrection/3994669-4475115, http://linkedlifedata.com/resource/pubmed/commentcorrection/3994669-4508129, http://linkedlifedata.com/resource/pubmed/commentcorrection/3994669-4508324, http://linkedlifedata.com/resource/pubmed/commentcorrection/3994669-4798146, http://linkedlifedata.com/resource/pubmed/commentcorrection/3994669-4821870, http://linkedlifedata.com/resource/pubmed/commentcorrection/3994669-4824925, http://linkedlifedata.com/resource/pubmed/commentcorrection/3994669-4850305, http://linkedlifedata.com/resource/pubmed/commentcorrection/3994669-4855726, http://linkedlifedata.com/resource/pubmed/commentcorrection/3994669-4883055, http://linkedlifedata.com/resource/pubmed/commentcorrection/3994669-4966930, http://linkedlifedata.com/resource/pubmed/commentcorrection/3994669-5102928, http://linkedlifedata.com/resource/pubmed/commentcorrection/3994669-512, http://linkedlifedata.com/resource/pubmed/commentcorrection/3994669-57805, http://linkedlifedata.com/resource/pubmed/commentcorrection/3994669-603622, http://linkedlifedata.com/resource/pubmed/commentcorrection/3994669-6160381, http://linkedlifedata.com/resource/pubmed/commentcorrection/3994669-6162459, http://linkedlifedata.com/resource/pubmed/commentcorrection/3994669-6176849, http://linkedlifedata.com/resource/pubmed/commentcorrection/3994669-6186911, http://linkedlifedata.com/resource/pubmed/commentcorrection/3994669-6189917, http://linkedlifedata.com/resource/pubmed/commentcorrection/3994669-6204636, http://linkedlifedata.com/resource/pubmed/commentcorrection/3994669-656053, http://linkedlifedata.com/resource/pubmed/commentcorrection/3994669-656054, http://linkedlifedata.com/resource/pubmed/commentcorrection/3994669-670193, http://linkedlifedata.com/resource/pubmed/commentcorrection/3994669-7295778, http://linkedlifedata.com/resource/pubmed/commentcorrection/3994669-73386, http://linkedlifedata.com/resource/pubmed/commentcorrection/3994669-86497, http://linkedlifedata.com/resource/pubmed/commentcorrection/3994669-88298, http://linkedlifedata.com/resource/pubmed/commentcorrection/3994669-91647, http://linkedlifedata.com/resource/pubmed/commentcorrection/3994669-925023, http://linkedlifedata.com/resource/pubmed/commentcorrection/3994669-999647
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0264-6021
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
226
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
477-85
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1985
pubmed:articleTitle
Surface-simulation synthesis of the substrate-binding site of an enzyme. Demonstration with trypsin.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't