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rdf:type |
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lifeskim:mentions |
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pubmed:dateCreated |
1985-6-19
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pubmed:abstractText |
We describe in vitro measurements of heteroduplex base mispaired intermediates involving 5-bromouracil and 2-aminopurine in A X T----G X C and G X C----A X T transition mutation pathways. For the case of 2-aminopurine, 2-aminopurine X cytosine mispairs are formed at a much higher frequency than adenine X cytosine mispairs in either transition pathway. For the case of 5-bromouracil, at least a 40-fold increase in 5-bromouracil X guanine mispairs are observed over thymine X guanine mispairs but only in the G X C----A X T pathway. In the A X T----G X C pathway, mispairs involving 5-bromouracil are formed 2.5-fold more frequently to those involving thymine suggesting perhaps that 5-bromouracil may exhibit substantially different base-pairing behavior depending on whether it is present as a template base or as a deoxyribonucleosides triphosphate substrate. The effect of the base analogs on dNTP pool size perturbations is discussed. A measurement of dNTP pools in 2-aminopurine mutagenized bacteriophage T4-infected cells is presented. An approximate eight-fold expansion in common dNTP pools is observed in a ts L141 antimutator genetic background compared to wild type T4 43+ and ts L56 mutator backgrounds. The effects of distorted dNTP pools on mutagenesis will be considered.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/2'-deoxycytidine 5'-triphosphate,
http://linkedlifedata.com/resource/pubmed/chemical/2-Aminopurine,
http://linkedlifedata.com/resource/pubmed/chemical/Adenine,
http://linkedlifedata.com/resource/pubmed/chemical/Bromouracil,
http://linkedlifedata.com/resource/pubmed/chemical/Cytosine,
http://linkedlifedata.com/resource/pubmed/chemical/Deoxycytosine Nucleotides,
http://linkedlifedata.com/resource/pubmed/chemical/Deoxyribonucleotides,
http://linkedlifedata.com/resource/pubmed/chemical/Deoxyuracil Nucleotides,
http://linkedlifedata.com/resource/pubmed/chemical/Guanine,
http://linkedlifedata.com/resource/pubmed/chemical/Nucleic Acid Heteroduplexes,
http://linkedlifedata.com/resource/pubmed/chemical/Thymine,
http://linkedlifedata.com/resource/pubmed/chemical/bromodeoxyuridine triphosphate
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pubmed:status |
MEDLINE
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pubmed:issn |
0090-5542
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
31
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
409-23
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:3994630-2-Aminopurine,
pubmed-meshheading:3994630-Adenine,
pubmed-meshheading:3994630-Base Composition,
pubmed-meshheading:3994630-Bromouracil,
pubmed-meshheading:3994630-Cytosine,
pubmed-meshheading:3994630-Deoxycytosine Nucleotides,
pubmed-meshheading:3994630-Deoxyribonucleotides,
pubmed-meshheading:3994630-Deoxyuracil Nucleotides,
pubmed-meshheading:3994630-Guanine,
pubmed-meshheading:3994630-Mutation,
pubmed-meshheading:3994630-Nucleic Acid Heteroduplexes,
pubmed-meshheading:3994630-Thymine
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pubmed:year |
1985
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pubmed:articleTitle |
The biochemical basis of 5-bromouracil- and 2-aminopurine-induced mutagenesis.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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