Linked Life Data

3994583

Source:http://linkedlifedata.com/resource/pubmed/id/3994583

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1985-6-7
pubmed:abstractText
Male mice of two inbred strains, C57BR/cdJ and CBA/J, were maintained either on an atherogenic diet rich in cholesterol (5%) and saturated fat (30%) or on a control Purina Laboratory Chow diet. After 3 weeks on a 90% atherogenic/10% chow diet, the C57BR/cdJ mice showed a fivefold increase in plasma total cholesterol levels, while the CBA/J strain indicated only a twofold increase. On the atherogenic diet, both groups of animals showed marked increases in a lipoprotein of a broad density spectrum (1.006 greater than d less than 1.063) with prebeta and beta mobility on agarose gel electrophoresis, but the C57BR/cdJ strain showed a greater increase than the CBA/J strain. The very low density lipoprotein (VLDL) contained predominantly lipoprotein of prebeta mobility, while intermediate density lipoprotein (IDL) and low density lipoprotein (LDL) contained lipoprotein of beta-mobility. In both strains, the major lipid component in this lipoprotein was cholesteryl ester and both strains showed large amounts of an apoprotein (apo) with E mobility as defined for known apoproteins by sodium dodecyl sulfate polyacrylamide gel electrophoresis or by isoelectric focusing on polyacrylamide-urea disc gels. The phosphatidylcholine/free cholesterol ratio of the VLDL, IDL, and LDL fractions isolated from the C57BR/cdJ mice on the atherogenic diet (0.76 to 0.95) was noticeably lower than that (1.10 to 1.19) from the CBA/J mice maintained on the same diet. There was a marked decrease in the high density lipoprotein (HDL) fraction in both strains of mice on the atherogenic diet, but a greater reduction in the HDL level of the C57BR/cdJ strain with a 50% decrease in the phosphatidylcholine/free cholesterol ratio.(ABSTRACT TRUNCATED AT 250 WORDS)
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0276-5047
pubmed:author
pubmed:issnType
Print
pubmed:volume
5
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
256-64
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:articleTitle
Lipoprotein levels in genetically selected mice with increased susceptibility to atherosclerosis.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't