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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1979-12-20
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pubmed:abstractText |
The possible role of pili in the pathogenesis of urinary tract infections caused by Klebsiella pneumoniae was studied in an in vitro mixture of a phosphate-buffered saline suspension of rat bladder epithelial cells and phosphate-buffered saline-washed K. pneumoniae. Nonpiliated and piliated populations derived from a single K. pneumoniae strain were obtained by controlling the total time of growth in broth medium. The piliated phase demonstrated a significant increase in adherence when compared to the nonpiliated phase. Incubation of the bacteria and epithelial cell mixture at 4 and 37 degrees C resulted in no differences in adherence; optimal adherence occurred at pH 5. Pretreatment of the bacteria with enzymes to destroy the pili resulted in a decrease in adherence, as did killing the bacteria by various means before adherence testing. Pretreatment of the epithelial cells with certain saccharides inhibited bacterial adherence. Finally, a 96% decrease in adherence was observed after coincubation of bacteria and epithelial cells with papain-treated antipili antibodies. Thus, it appears that pili on the surface of K. pneumoniae mediate attachment of the bacteria to rat bladder epithelial cells.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/39895-13295908,
http://linkedlifedata.com/resource/pubmed/commentcorrection/39895-13367987,
http://linkedlifedata.com/resource/pubmed/commentcorrection/39895-13587981,
http://linkedlifedata.com/resource/pubmed/commentcorrection/39895-13818511,
http://linkedlifedata.com/resource/pubmed/commentcorrection/39895-14489301,
http://linkedlifedata.com/resource/pubmed/commentcorrection/39895-21933,
http://linkedlifedata.com/resource/pubmed/commentcorrection/39895-22493,
http://linkedlifedata.com/resource/pubmed/commentcorrection/39895-336832,
http://linkedlifedata.com/resource/pubmed/commentcorrection/39895-361565,
http://linkedlifedata.com/resource/pubmed/commentcorrection/39895-361629,
http://linkedlifedata.com/resource/pubmed/commentcorrection/39895-361900,
http://linkedlifedata.com/resource/pubmed/commentcorrection/39895-4200266,
http://linkedlifedata.com/resource/pubmed/commentcorrection/39895-4556971,
http://linkedlifedata.com/resource/pubmed/commentcorrection/39895-4610081,
http://linkedlifedata.com/resource/pubmed/commentcorrection/39895-4892377,
http://linkedlifedata.com/resource/pubmed/commentcorrection/39895-5318403,
http://linkedlifedata.com/resource/pubmed/commentcorrection/39895-794477
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0019-9567
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
25
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
729-37
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:39895-Adhesiveness,
pubmed-meshheading:39895-Animals,
pubmed-meshheading:39895-Antigen-Antibody Reactions,
pubmed-meshheading:39895-Epithelium,
pubmed-meshheading:39895-Female,
pubmed-meshheading:39895-Fimbriae, Bacterial,
pubmed-meshheading:39895-Hydrogen-Ion Concentration,
pubmed-meshheading:39895-Klebsiella pneumoniae,
pubmed-meshheading:39895-Male,
pubmed-meshheading:39895-Mannose,
pubmed-meshheading:39895-Mannosides,
pubmed-meshheading:39895-Rats,
pubmed-meshheading:39895-Temperature,
pubmed-meshheading:39895-Urinary Bladder
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pubmed:year |
1979
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pubmed:articleTitle |
Evidence for pili-mediated adherence of Klebsiella pneumoniae to rat bladder epithelial cells in vitro.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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