Linked Life Data

3987861

Source:http://linkedlifedata.com/resource/pubmed/id/3987861

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1985-5-28
pubmed:abstractText
Adult human biopsied muscle grown de novo from myoblasts in monolayer was cocultured with spinal cord explants of 13- to 14-day-old rat embryos. Cocultures were established 15 days after myoblast fusion. Autoradiography of 125I-alpha-bungarotoxin and acetylcholinesterase staining were carried out on 27- to 56-day-old muscle-cord cocultures. Large clusters of 125I-alpha-bungarotoxin, indicating clusters of acetylcholine receptors, were present at nerve-muscle contacts but not elsewhere on the muscle fibers. Accumulation of acetylcholinesterase was also present at nerve-muscle contacts. This study demonstrates that monolayer cultured adult human muscle can be innervated by embryonic rat spinal cord neurons and thus there is no requirement for an original basal lamina tube as it is present when organ-cultured human muscle is innervated.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0014-4886
pubmed:author
pubmed:issnType
Print
pubmed:volume
88
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
327-35
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1985
pubmed:articleTitle
Acetylcholine receptors and acetylcholinesterase accumulate at the nerve-muscle contacts of de novo grown human monolayer muscle cocultured with fetal rat spinal cord.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.