Linked Life Data

3986665

Source:http://linkedlifedata.com/resource/pubmed/id/3986665

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1985-6-19
pubmed:abstractText
Platelet-derived growth factor (PDGF) was isolated from platelets obtained from fresh bovine blood. The platelet lysates were extracted with acid-ethanol, according to the classic procedure that has been applied to the preparation of various protein hormones and transforming growth factors, but not until now to PDGF isolation. Bovine PDGF was further purified by ion-exchange chromatography on DEAE-Sephadex, CM-Sephadex, and finally by molecular sieving on Sephadex G-100. Bovine PDGF has an isoelectric point of 9.45-10.6 and a molecular weight ranging from 28 000 to 31 000. Reduction of bovine PDGF with 2-mercaptoethanol revealed the presence of two polypeptides with molecular weights of approximately 14 000 and 15 000. A simple biologic microassay of cell multiplication-stimulating activity has been developed in our laboratory. Purified bovine PDGF stimulated the proliferation of BALB/c 3T3 or human fibroblasts at minimal concentrations of 0.36-1.5 ng/mL.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0714-7511
pubmed:author
pubmed:issnType
Print
pubmed:volume
63
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
187-94
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1985
pubmed:articleTitle
Properties of purified bovine platelet-derived growth factor stimulating proliferation of human and mouse fibroblasts.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't