Linked Life Data

3986273

Source:http://linkedlifedata.com/resource/pubmed/id/3986273

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1985-6-12
pubmed:abstractText
Ovariectomized rats were treated with estradiol for 3 days after which their uteri were incubated in vitro and radioactive media proteins were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Media were also chromatographed on G-25 Sephadex Blue Sepharose columns to isolate subsets of proteins. The results demonstrate that two proteins are consistently increased following estrogen treatment. These proteins have molecular weights of 104,000 and 65,000. Neither protein binds to Blue Sepharose to a great extent. The use of the protein synthesis inhibitors, emetine and actinomycin D, demonstrates that the proteins are synthesized de novo. These two proteins may serve as markers for genomic response to estradiol in the rat uterus.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0006-3363
pubmed:author
pubmed:issnType
Print
pubmed:volume
32
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
443-50
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1985
pubmed:articleTitle
Comparison of media proteins from ovariectomized rat uteri following estrogen treatment.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't