Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1985-5-8
pubmed:abstractText
The pyrogallol autoxidation assay for superoxide dismutase was adapted for analysis by centrifugal analyzer. The precision of the enzymatic assay was estimated using bovine blood superoxide dismutase. Specific activity determined for human erythrocyte superoxide dismutase by the procedure agreed with previously reported determinations. Recovery of bovine blood superoxide dismutase from hemolysates provided a measure of proportional analytic error. Difficulties inherent in attempting to spectrophotometrically assay an enzyme catalyzing a reaction requiring a free radical substrate cannot be eliminated by an automated method. However, the centrifugal analyzer method makes routine enzymatic analysis for superoxide dismutase in numerous samples technically feasible. Moreover, automated analysis can conveniently include a standard such as bovine blood superoxide dismutase when enzyme levels in tissue extracts are assayed. These advantages make the method for assay of superoxide dismutase enzyme activity a useful procedure.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0146-4760
pubmed:author
pubmed:issnType
Print
pubmed:volume
9
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
36-9
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:articleTitle
An automated analysis for superoxide dismutase enzyme activity.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S.