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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1985-3-6
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pubmed:abstractText |
Ten murine monoclonal antibodies have been produced that are specific for bovine milk lipoprotein lipase. One monoclonal antibody, bLPL-mAb-7, inhibited completely the apolipoprotein C-II (apo-C-II)-dependent enzymic hydrolysis of trioleoylglycerol in a phospholipid-stabilized emulsion, but had no effect on the hydrolysis of the water-soluble substrate p-nitro-phenylacetate. Four times more bLPL-mAb-7 was required to achieve 50% inactivation of lipoprotein lipase activity when the enzyme was preincubated with excess apo-C-II. Disruption of the binding of a dansyl-labeled apo-C-II peptide to lipoprotein lipase by bLPL-mAb-7 was demonstrated by resonance energy transfer, both in the presence and absence of lipid. This antibody thus appears to recognize the apo-C-II binding site of lipoprotein lipase. In addition, bLPL-mAb-7 also inhibited the lipoprotein lipase activity of human post-heparin plasma.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
25
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pubmed:volume |
260
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
893-8
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:3968071-Animals,
pubmed-meshheading:3968071-Antibodies, Monoclonal,
pubmed-meshheading:3968071-Antibody Specificity,
pubmed-meshheading:3968071-Apolipoprotein C-II,
pubmed-meshheading:3968071-Apolipoproteins C,
pubmed-meshheading:3968071-Binding Sites,
pubmed-meshheading:3968071-Energy Transfer,
pubmed-meshheading:3968071-Heparin,
pubmed-meshheading:3968071-Hydrolysis,
pubmed-meshheading:3968071-Lipoprotein Lipase,
pubmed-meshheading:3968071-Male,
pubmed-meshheading:3968071-Mice,
pubmed-meshheading:3968071-Mice, Inbred BALB C,
pubmed-meshheading:3968071-Milk,
pubmed-meshheading:3968071-Nitrophenols,
pubmed-meshheading:3968071-Triglycerides
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pubmed:year |
1985
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pubmed:articleTitle |
Monoclonal antibodies against bovine milk lipoprotein lipase. Characterization of an antibody specific for the apolipoprotein C-II binding site.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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