Linked Life Data

3968066

Source:http://linkedlifedata.com/resource/pubmed/id/3968066

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1985-3-6
pubmed:abstractText
The metabolism of type I procollagen mRNAs was studied in confluent cultures of human skin fibroblasts using the recombinant plasmids Hf677 and Hf32, which contain DNA sequences complementary to human pro-alpha 1(I) and pro-alpha 2(I) mRNAs, respectively. The ratio for the amount of pro-alpha 1(I) mRNAs to pro-alpha 2(I) mRNAs was 2.06:1. These mRNAs were labeled during in vitro transcription of isolated nuclei in the ratio 2.16:1 and during a pulse in cellulo in the ratio 1.96:1, suggesting that the two species are synthesized in the ratio 2:1. No significant differences were found in the rates of degradation, the half-lives of the pro-alpha 1(I) and pro-alpha 2(I) mRNAs being 9.2 and 8.4 h, respectively. Addition of 10 microM cortisol to the growth medium reduced the amounts of the type I procollagen mRNAs by half in 6 h, leaving their ratio unaltered. Incubation of the cells with cortisol had only minor effects on the subsequent synthesis of the mRNAs in isolated nuclei in vitro, whereas the degradation rates in cellulo were distinctly accelerated.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
25
pubmed:volume
260
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
720-5
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1985
pubmed:articleTitle
Synthesis and degradation of type I procollagen mRNAs in cultured human skin fibroblasts and the effect of cortisol.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't