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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1985-2-5
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pubmed:abstractText |
Hyperimmune, but not normal immune, monospecific antiserum made to capsid protein of Sindbis virus (SIN) was found to cause cytolysis equally well of both SIN- and Semliki Forest virus-infected L929 cells in antibody-dependent, complement-mediated cytotoxicity assays. The cell surface reactivity of the hyperimmune antiserum was also demonstrated by solid-phase radioimmune assays with unfixed infected cells or infected cells fixed with low concentrations of glutaraldehyde (0.025%) before reactivity with antisera. Higher concentrations of glutaraldehyde lowered the sensitivity of detection. Purified SIN capsid protein specifically inhibited antibody-dependent, complement-mediated cytotoxicity by the monospecific anti-capsid protein serum on SIN- and Semliki Forest virus-infected target cells. That hyperimmune anti-SIN serum also cross-reacts with capsid protein on the surface of Semliki Forest virus-infected cells was suggested by the fact that capsid protein inhibited cross-cytolysis in the antibody-dependent, complement-mediated cytotoxicity assay. The latter antiserum was collected after repeated injections of purified virions over a 9-month period. The results suggest that hyperimmune monospecific antisera made to SIN capsid protein or hyperimmune antisera to SIN or Semliki Forest virions detect homologous and cross-reacting capsid protein determinants on the surface of infected cells.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3965743-1067601,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3965743-1159895,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3965743-1167945,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3965743-1182802,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3965743-14450081,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3965743-14907713,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3965743-166500,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3965743-171418,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3965743-233901,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3965743-302303,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3965743-388439,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3965743-4373739,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3965743-4472532,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3965743-4675142,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3965743-4711106,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3965743-473598,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3965743-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3965743-54978,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3965743-58459,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3965743-59785,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3965743-6154376,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3965743-6172382,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3965743-6177636,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3965743-6181415,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3965743-6198430,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/3965743-6271974,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/3965743-6286760,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3965743-6694261,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3965743-6767810,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/3965743-6941270,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3965743-6985476,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3965743-70496,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3965743-7077663,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3965743-7236011,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3965743-726255,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3965743-7365868,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3965743-837448,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3965743-840296,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3965743-875134
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0022-538X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
53
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
198-204
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:3965743-Animals,
pubmed-meshheading:3965743-Antigen-Antibody Complex,
pubmed-meshheading:3965743-Capsid,
pubmed-meshheading:3965743-Cell Transformation, Viral,
pubmed-meshheading:3965743-Chick Embryo,
pubmed-meshheading:3965743-Cross Reactions,
pubmed-meshheading:3965743-Cytotoxicity, Immunologic,
pubmed-meshheading:3965743-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:3965743-Fibroblasts,
pubmed-meshheading:3965743-Immune Sera,
pubmed-meshheading:3965743-L Cells (Cell Line),
pubmed-meshheading:3965743-Mice,
pubmed-meshheading:3965743-Radioimmunoassay,
pubmed-meshheading:3965743-Sindbis Virus
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pubmed:year |
1985
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pubmed:articleTitle |
Detection of immunologically cross-reacting capsid protein of alphaviruses on the surfaces of infected L929 cells.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.
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