Linked Life Data

3961734

Source:http://linkedlifedata.com/resource/pubmed/id/3961734

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1986-5-15
pubmed:abstractText
Abnormal platelet aggregation seen in experimentally induced diabetic, hypercholesterolemic and spontaneously hypertensive rats (SHR) has been linked with increased prostaglandin synthesis. The present study was conducted to examine the role of prostaglandins in rat platelet activation using normal Wistar Kyoto (WKY) and SHR rats. Up to 30 microM ADP did not induce secondary phase of platelet aggregation in rat PRP and up to 30 microM epinephrine did not produce any response in rat PRP. In other experiments ADP (1.0 microM) and epinephrine (2.0 microM) induced typical biphasic aggregation responses in human PRP. Up to 20 microM U46619, a stable analog of prostaglandin H2, did not induce platelet aggregation in rat PRP or washed rat platelets. In contrast 2.0 microM U46619 caused maximal aggregation in human PRP and washed human platelets. Arachidonic acid (1.5-2.0 mM) induced aggregation in washed rat platelets. However, this was associated with excessive (67% and 94%) loss of cytoplasmic LDH. The low concentrations of thrombin (0.04 and 0.05 U/ml), induced two to three-fold increase in aggregation response in SHR platelets as compared to WKY platelets. Higher concentrations of thrombin (0.1 and 0.3 U/ml) induced similar aggregation responses in SHR and WKY platelets. Thrombin (0.04-0.3 U/ml) induced serotonin secretion in a concentration dependent manner. The extent of secretion was the same in SHR and WKY platelets at all concentrations. Thrombin-induced synthesis of thromboxane A2 (TXA2) in WKY and SHR platelets was quantified using a radioimmunoassay for TXB2. Thrombin (0.04-0.3 U/ml) produced TXB2 in WKY and SHR platelets in a concentration dependent manner. The SHR platelets produced significantly larger amounts of TXB2 as compared to WKY platelets. In other experiments aspirin (500 microM) inhibited thrombin (0.05 U/ml) induced TXB2 synthesis by 75% in both WKY and SHR platelets but failed to inhibit aggregation or secretion in either WKY or SHR platelets. Based on these data it is suggested that: (a) rat platelets inspite of their ability to synthesize TXA2 do not require TXA2 for aggregation; and (b) the rat may not be an appropriate model to study the role of prostaglandins in normal or abnormal platelet aggregation.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0049-3848
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
41
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
555-66
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:3961734-Adenosine Diphosphate, pubmed-meshheading:3961734-Animals, pubmed-meshheading:3961734-Aspirin, pubmed-meshheading:3961734-Blood Platelets, pubmed-meshheading:3961734-Blood Pressure, pubmed-meshheading:3961734-Disease Models, Animal, pubmed-meshheading:3961734-Epinephrine, pubmed-meshheading:3961734-Humans, pubmed-meshheading:3961734-Hypertension, pubmed-meshheading:3961734-Kinetics, pubmed-meshheading:3961734-L-Lactate Dehydrogenase, pubmed-meshheading:3961734-Platelet Aggregation, pubmed-meshheading:3961734-Prostaglandins, pubmed-meshheading:3961734-Rats, pubmed-meshheading:3961734-Rats, Inbred SHR, pubmed-meshheading:3961734-Rats, Inbred WKY, pubmed-meshheading:3961734-Serotonin, pubmed-meshheading:3961734-Species Specificity, pubmed-meshheading:3961734-Thrombin
pubmed:year
1986
pubmed:articleTitle
Evidence that the rat is not an appropriate model to study the role of prostaglandins in normal or abnormal platelet aggregation.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't