Linked Life Data

3956263

Source:http://linkedlifedata.com/resource/pubmed/id/3956263

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1986-4-25
pubmed:abstractText
Tumorigenicity assays of 48 intraspecies hybrids between human carcinoma-derived cells of the D98AH2 (HeLa) cell line and normal human diploid cells revealed that most are nontumorigenic in nude mice. Chromosome analysis revealed that these hybrids contained four chromosomes 11, presumably two from each parental cell. Their tumorigenic segregants sometimes, and their tumors always, had lost one or two copies of chromosome 11 (Klinger and Kaelbling, 1986). In this report we present evidence from analyses of DNA restriction fragment length polymorphic (RFLP) markers for the parental cell origin of the 11 which confirms that the four chromosomes 11 of the nontumorigenic hybrids consisted of two from each cell parent, and most notably, that one No. 11 of the diploid parental cell is always absent in cells of tumors that arise when the tumorigenic hybrids are injected into nude mice. We also found that both Nos. 11 of the D98 cells are identical at many RFLP sites on the short arm suggesting that loss of heterozygosity of the 11, or at least of the short arm of the 11, had occurred. Chromosome 11 of the diploid cells thus appears to carry alleles that suppress the tumorigenic potential of the D98 cells when present in two copies, but not in one. It remains to be seen if other chromosomes of the diploid cell effect suppression in concert with the 11.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:issn
0301-0171
pubmed:author
pubmed:issnType
Print
pubmed:volume
41
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
65-70
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1986
pubmed:articleTitle
Suppression of tumorigenicity in somatic cell hybrids. III. Cosegregation of human chromosome 11 of a normal cell and suppression of tumorigenicity in intraspecies hybrids of normal diploid x malignant cells.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.