Linked Life Data

3955665

Source:http://linkedlifedata.com/resource/pubmed/id/3955665

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1986-5-9
pubmed:abstractText
A heat shock-resistant variant of Chinese hamster cell line (CHO) was isolated from ethane methane sulfonate-treated CHO cells through selection by repeated exposures to elevated temperature. The variant, designated HR-01, was one to two order of magnitudes more resistant to lethal heat shock (46.0 degrees C) than the parental CHO strain (p-CHO). The heat shock resistance characteristic of this variant was stable. In addition, the HR-01 variant showed more elongated cell morphology, and was more adherent to substrate than p-CHO. When total proteins of p-CHO and HR-01 cells were compared in two-dimensional polyacrylamide gel electrophoresis, HSP90, a heat shock protein of Mr 90,000, was found to be the only protein that was expressed at a significantly higher level in HR-01 cells than in p-CHO cells. Because of the known intriguing molecular properties of HSP90, the HR-01 variant would be useful for further investigation of functions of HSP90 as well as the mechanism of acquiring heat shock resistance in mammalian cells.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0386-7196
pubmed:author
pubmed:issnType
Print
pubmed:volume
11
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
65-73
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
1986
pubmed:articleTitle
A heat shock-resistant variant of Chinese hamster cell line constitutively expressing heat shock protein of Mr 90,000 at high level.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't