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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
1986-5-22
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pubmed:abstractText |
Placental tissue with macroscopic visible vesicles was processed for chromosome analysis. Enzymatic tissue dissociation, culture initiation in Chang's medium, and final culture in a medium with high glucose content were used, which increased the success rate for karyotyping from 55% to 93%. Median culture time necessary for obtaining a karyotype decreased to about 1 week, irrespective of the chromosomal constitution. This was achieved in spite of increasing time for transportation. The metaphases obtained permitted detailed analysis of chromosomal polymorphisms. In 8 of 44 cases, the karyotype was established on short-term culture alone, which proved to be a valuable supplement in this study.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0165-4608
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
22
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
19-27
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:3955531-Cells, Cultured,
pubmed-meshheading:3955531-Chromosome Aberrations,
pubmed-meshheading:3955531-Chromosome Banding,
pubmed-meshheading:3955531-Female,
pubmed-meshheading:3955531-Humans,
pubmed-meshheading:3955531-Hydatidiform Mole,
pubmed-meshheading:3955531-Karyotyping,
pubmed-meshheading:3955531-Placenta,
pubmed-meshheading:3955531-Pregnancy,
pubmed-meshheading:3955531-Specimen Handling,
pubmed-meshheading:3955531-Time Factors,
pubmed-meshheading:3955531-Uterine Neoplasms
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pubmed:year |
1986
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pubmed:articleTitle |
Hydatidiform moles: methods for culture and cytogenetic analyses.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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