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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
1986-4-10
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pubmed:abstractText |
The metabolism of the two methionine precursors, L-homocysteine and 5'-deoxy-5'-methylthioadenosine was compared to the ability of these compounds to support cell growth in a Met-free medium, in the following mammalian cell lines: Raji, CCL 39 and BHK cells. These three cell lines metabolized L-homocysteine and 5'-deoxy-5'-methylthioadenosine into methionine, S-adenosyl-L-methionine and proteins. However there was a discrepancy between metabolic and growth studies: Raji cells could grow on L-homocysteine and on 5'-deoxy-5'-methylthioadenosine, BHK cells could grow on L-homocysteine but not on 5'-deoxy-5'-methylthioadenosine, and CCL 39 cells could not grow either on L-homocysteine or on 5'-deoxy-5'-methylthioadenosine. The metabolism of exogenous methionine, and of methionine endogenously synthesized from 5'-deoxy-5'-methylthioadenosine was studied in CCL 39 and Raji cells, incubated with 25 microM [methyl-14c] methionine + 25 microM 5'-deoxy-5'-methylthioadenosine or 25 microM [methyl-14c] 5'-deoxy-5'-methylthioadenosine + 25 microM methionine: there was no difference between the metabolism of exogenous and endogenous methionine in either type of cell. Our results indicate that i) "methionine dependence" initially described for L-homocysteine [Hoffman, R.M. and Erbe, R.W. (1976) Proc. Natl. Acad. Sci. USA 73, 1523], can also be observed with the other precursor of methionine, ie 5'-deoxy-5'-methylthioadenosine; ii) "methionine-dependence" can not be considered as the inability of a cell to grow on methionine endogenously synthesized from a precursor, but depends on the precursor used, and possibly, on a toxic effect of the precursor in the absence of methionine.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/5'-methylthioadenosine,
http://linkedlifedata.com/resource/pubmed/chemical/Adenosine,
http://linkedlifedata.com/resource/pubmed/chemical/Culture Media,
http://linkedlifedata.com/resource/pubmed/chemical/Deoxyadenosines,
http://linkedlifedata.com/resource/pubmed/chemical/Homocysteine,
http://linkedlifedata.com/resource/pubmed/chemical/Methionine,
http://linkedlifedata.com/resource/pubmed/chemical/Thionucleosides
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0006-291X
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
26
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pubmed:volume |
135
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
131-8
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pubmed:dateRevised |
2005-11-17
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pubmed:meshHeading |
pubmed-meshheading:3954761-Adenosine,
pubmed-meshheading:3954761-Animals,
pubmed-meshheading:3954761-Cell Division,
pubmed-meshheading:3954761-Cell Line,
pubmed-meshheading:3954761-Cricetinae,
pubmed-meshheading:3954761-Culture Media,
pubmed-meshheading:3954761-Deoxyadenosines,
pubmed-meshheading:3954761-Homocysteine,
pubmed-meshheading:3954761-Humans,
pubmed-meshheading:3954761-Methionine,
pubmed-meshheading:3954761-Thionucleosides
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pubmed:year |
1986
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pubmed:articleTitle |
Salvage of 5'-deoxy-5'-methylthioadenosine and L-homocysteine into methionine in cells cultured in a methionine-free medium: a study of "methionine-dependence".
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pubmed:publicationType |
Journal Article
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