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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1986-3-31
|
| pubmed:abstractText |
The most commonly used method for quantifying D-glucaric acid, the enzyme inhibition method, is a two-step procedure. In the first step of the present study, urine was boiled at acidic pH to convert glucaric acid to 1,4-glucarolactone, which inhibits beta-glucuronidase activity. In the second step, inhibition of beta-glucuronidase by the prepared urine was measured. This is an indirect measure of the D-glucaric acid concentration. The second step was adapted to a Cobas-Bio centrifugal analyzer. Values on a reference population obtained by the manual method were highly correlated with values obtained by the semiautomated method described here (r = 0.976, p = 0.25), and precision was comparable by the two methods. The throughput increased from 8 to 20 samples per day when the automated method was used.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:issn |
0146-4760
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
10
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
15-7
|
| pubmed:dateRevised |
2004-11-17
|
| pubmed:meshHeading |
pubmed-meshheading:3951202-Autoanalysis,
pubmed-meshheading:3951202-Glucaric Acid,
pubmed-meshheading:3951202-Glucuronidase,
pubmed-meshheading:3951202-Humans,
pubmed-meshheading:3951202-Hydrogen-Ion Concentration,
pubmed-meshheading:3951202-Indicators and Reagents,
pubmed-meshheading:3951202-Sugar Acids,
pubmed-meshheading:3951202-Ultracentrifugation
|
| pubmed:articleTitle |
A semiautomated procedure for urinary D-glucaric acid using a centrifugal analyzer.
|
| pubmed:publicationType |
Journal Article
|