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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3
|
pubmed:dateCreated |
1986-4-3
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pubmed:abstractText |
An enzymatic method for the calibration of a turbidimetric lipase assay is described, based on measurement of free fatty acids liberated by the action of lipase. The substrate of the turbidimetric assay is a colipase-containing triolein emulsion. For determination of the free fatty acids a commercial test kit including acyl-CoA synthetase, acyl-CoA oxidase, and peroxidase is used. Intra- and interassay imprecision (CV) is about 5% at above-normal lipase activities, about 10% at normal values. Temperature coefficients are 1.24 and 1.45, respectively, for measurements at 30 and 37 degrees C vs 25 degrees C.
|
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
|
pubmed:month |
Mar
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pubmed:issn |
0009-9147
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
32
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
545-7
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pubmed:dateRevised |
2004-11-17
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pubmed:meshHeading |
pubmed-meshheading:3948404-Animals,
pubmed-meshheading:3948404-Calibration,
pubmed-meshheading:3948404-Fatty Acids, Nonesterified,
pubmed-meshheading:3948404-Humans,
pubmed-meshheading:3948404-Lipase,
pubmed-meshheading:3948404-Nephelometry and Turbidimetry,
pubmed-meshheading:3948404-Swine,
pubmed-meshheading:3948404-Temperature
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pubmed:year |
1986
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pubmed:articleTitle |
An enzymatic method for calibration of serum lipase assays.
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pubmed:publicationType |
Journal Article
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