Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1986-4-3
pubmed:abstractText
An enzymatic method for the calibration of a turbidimetric lipase assay is described, based on measurement of free fatty acids liberated by the action of lipase. The substrate of the turbidimetric assay is a colipase-containing triolein emulsion. For determination of the free fatty acids a commercial test kit including acyl-CoA synthetase, acyl-CoA oxidase, and peroxidase is used. Intra- and interassay imprecision (CV) is about 5% at above-normal lipase activities, about 10% at normal values. Temperature coefficients are 1.24 and 1.45, respectively, for measurements at 30 and 37 degrees C vs 25 degrees C.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0009-9147
pubmed:author
pubmed:issnType
Print
pubmed:volume
32
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
545-7
pubmed:dateRevised
2004-11-17
pubmed:meshHeading
pubmed:year
1986
pubmed:articleTitle
An enzymatic method for calibration of serum lipase assays.
pubmed:publicationType
Journal Article