Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1986-2-20
pubmed:abstractText
We present a genetic and physical characterization of the IncM plasmid pBWH1. A physical map was constructed for the enzymes EcoRI, BamHI, SalI, BglII, HindIII, MstII, and XhoI. A series of deletions and a series of subclones of pBWH1 were constructed and used to determine the locations on this map of the transfer region; the replication region; and the genes determining resistance to beta-lactams, chloramphenicol, the sulfonamides, and gentamicin. We compared 51 different isolates, including isolates which had lost individual antibiotic resistances or the transfer phenotype, and showed that variations occurred in all regions of the plasmid genome. Frequently, correlations could be made between phenotypic variation and variation of the EcoRI fragments which contained the gene determining that phenotype.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/3941051-1094460, http://linkedlifedata.com/resource/pubmed/commentcorrection/3941051-1267736, http://linkedlifedata.com/resource/pubmed/commentcorrection/3941051-2987996, http://linkedlifedata.com/resource/pubmed/commentcorrection/3941051-307230, http://linkedlifedata.com/resource/pubmed/commentcorrection/3941051-327269, http://linkedlifedata.com/resource/pubmed/commentcorrection/3941051-368583, http://linkedlifedata.com/resource/pubmed/commentcorrection/3941051-384425, http://linkedlifedata.com/resource/pubmed/commentcorrection/3941051-388356, http://linkedlifedata.com/resource/pubmed/commentcorrection/3941051-6243621, http://linkedlifedata.com/resource/pubmed/commentcorrection/3941051-6251029, http://linkedlifedata.com/resource/pubmed/commentcorrection/3941051-6267312, http://linkedlifedata.com/resource/pubmed/commentcorrection/3941051-6294054, http://linkedlifedata.com/resource/pubmed/commentcorrection/3941051-6306395, http://linkedlifedata.com/resource/pubmed/commentcorrection/3941051-6348505, http://linkedlifedata.com/resource/pubmed/commentcorrection/3941051-6757994, http://linkedlifedata.com/resource/pubmed/commentcorrection/3941051-6783706, http://linkedlifedata.com/resource/pubmed/commentcorrection/3941051-7396449
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0021-9193
pubmed:author
pubmed:issnType
Print
pubmed:volume
165
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
47-52
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1986
pubmed:articleTitle
Genetic and physical characterization of IncM plasmid pBWH1 and its variance among natural isolates.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.