Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1986-5-23
pubmed:abstractText
Highly purified synthetic salmonid melanin concentrating hormone (MCH) and some analogs were investigated for their ability to concentrate the pigment in scale melanophores of the Chinese grass carp, Ctenopharyngodon idellus, to produce melanin dispersion in frog or lizard melanophores and to inhibit alpha-MSH in its action on mouse melanoma and rat adrenal glomerulosa cells in vitro. In the grass carp, MCH produced half-maximal pigment aggregation at 6 X 10(-11) M and its oxidized form at 7 X 10(-11) M. Replacement of the two methionines at position 3 and 6 with norvaline lowered the potency by a factor of 2.7 and with propargylglycine by a factor of about 7. Linear, Cys5,14-Acm-protected MCH was a full agonist of MCH but with a 345-fold lower potency. Iodinated MCH showed similar, low activity. In tetrapods, salmonid MCH and its analogs displayed only marginal pigment dispersion at concentrations greater than 10(-5) M. Alkali-treatment of MCH increased the pigment-dispersing potency by a factor of about 30 whereas the activity for pigment aggregation in the grass carp was destroyed. At high concentrations (10(-6), 10(-5) M) MCH also stimulated tyrosinase activity in B-16 mouse melanoma cells but did not modify the effects of alpha-MSH in this system. By contrast, when tested on rat adrenal glomerulosa cells, salmonid MCH had no effect alone but at a concentration of greater than 10(-10) M it slightly reduced corticosterone production by an alpha-MSH concentration of 10(-7) M. Aldosterone production was not affected and MCH did not influence the response to ACTH.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0196-9781
pubmed:author
pubmed:issnType
Print
pubmed:volume
6
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1125-30
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:3938841-Adrenal Cortex, pubmed-meshheading:3938841-Aldosterone, pubmed-meshheading:3938841-Amino Acid Sequence, pubmed-meshheading:3938841-Animals, pubmed-meshheading:3938841-Biological Assay, pubmed-meshheading:3938841-Carps, pubmed-meshheading:3938841-Cell Line, pubmed-meshheading:3938841-Corticosterone, pubmed-meshheading:3938841-Hypothalamic Hormones, pubmed-meshheading:3938841-Lizards, pubmed-meshheading:3938841-Melanins, pubmed-meshheading:3938841-Melanoma, pubmed-meshheading:3938841-Melanophores, pubmed-meshheading:3938841-Mice, pubmed-meshheading:3938841-Monophenol Monooxygenase, pubmed-meshheading:3938841-Pigments, Biological, pubmed-meshheading:3938841-Pituitary Hormones, pubmed-meshheading:3938841-Rana pipiens, pubmed-meshheading:3938841-Rats, pubmed-meshheading:3938841-Skin, pubmed-meshheading:3938841-Structure-Activity Relationship
pubmed:articleTitle
Effect of melanin concentrating hormone on pigment and adrenal cells in vitro.
pubmed:publicationType
Journal Article, In Vitro, Research Support, Non-U.S. Gov't