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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
13A
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pubmed:dateCreated |
1986-4-14
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pubmed:abstractText |
Direct sequencing of mRNA has shown that the early primary response of the BALB/c mouse to the hapten 2-phenyloxazolone is dominated by antibodies with a particular light chain, V kappa-Ox1. Although the V kappa-Ox1 sequence is still commonly expressed later in the response it now includes a number of nucleotide changes. From two independent BALB/c germ-line DNA libraries 13 different genes hybridizing to a V kappa-Ox1 probe were isolated and characterized. Two are identical to mRNA sequences found in the early primary response, one of which is the V kappa-Ox1 sequence. None of the germ-line clones show the characteristic nucleotide changes contained in the late anti-phenyloxazolone light chain mRNAs. These results demonstrate that the V kappa-Ox1 sequence used in the early primary response is entirely encoded by the germ-line and further substantiate the importance of somatic mutations in the maturation of the anti-phenyloxazolone response. The statistical analysis of the data shows that the V kappa-Ox1 related germ-line gene family contains greater than 20 and probably less than 50 genes.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3937727-106394,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3937727-115000,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3937727-2985470,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3937727-3924600,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3937727-3927006,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3937727-3927173,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3937727-6285297,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3937727-6286818,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3937727-6298778,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3937727-6300689,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3937727-6310323,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3937727-6401310,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3937727-6420501,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3937727-6432337,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3937727-6504141,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3937727-6546718,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3937727-6795591,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3937727-6795638,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3937727-6799208,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3937727-6803241,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3937727-6806376,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3937727-6877353,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3937727-7162519,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3937727-7300683,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3937727-7350544
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0261-4189
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
16
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pubmed:volume |
4
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3439-45
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:3937727-Animals,
pubmed-meshheading:3937727-Antibody Diversity,
pubmed-meshheading:3937727-Cloning, Molecular,
pubmed-meshheading:3937727-DNA,
pubmed-meshheading:3937727-Immunoglobulin Variable Region,
pubmed-meshheading:3937727-Immunoglobulin kappa-Chains,
pubmed-meshheading:3937727-Mice,
pubmed-meshheading:3937727-Mutation,
pubmed-meshheading:3937727-Oxazoles,
pubmed-meshheading:3937727-Oxazolone
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pubmed:year |
1985
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pubmed:articleTitle |
Light chain germ-line genes and the immune response to 2-phenyloxazolone.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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