Linked Life Data

3935321

Source:http://linkedlifedata.com/resource/pubmed/id/3935321

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2 Pt 1
pubmed:dateCreated
1986-2-12
pubmed:abstractText
We have introduced four point mutations into the 5' junction (GTATGT) and TACTAAC box of a yeast intron-containing gene coding for beta-galactosidase. To analyze quantitatively mutant combinations, we deliberately avoided nucleotides adjacent to the sites of cleavage (GTATGT) and lariat formation (TACTAAC) and chose positions with expected weak effects on splicing. All four mutants do not affect, or only marginally affect, gene expression. The levels of precursor RNA and intermediates indicate, however, that splicing efficiency is affected in all cases; the first stage of splicing, 5' cleavage and lariat formation, is reduced two to 15-fold in these four mutants. The mutants were combined to generate compensatory and noncompensatory double mutant combinations. No evidence for a specific base-pairing interaction between the 5' junction and TACTAAC box could be obtained. The results suggest that all conserved positions in the 5' junction and TACTAAC box play a role in splicing efficiency.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0092-8674
pubmed:author
pubmed:issnType
Print
pubmed:volume
43
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
423-30
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1985
pubmed:articleTitle
A quantitative analysis of the effects of 5' junction and TACTAAC box mutants and mutant combinations on yeast mRNA splicing.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.