| pubmed-article:3934284 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:3934284 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:3934284 | lifeskim:mentions | umls-concept:C0020792 | lld:lifeskim |
| pubmed-article:3934284 | lifeskim:mentions | umls-concept:C0022688 | lld:lifeskim |
| pubmed-article:3934284 | lifeskim:mentions | umls-concept:C0003241 | lld:lifeskim |
| pubmed-article:3934284 | lifeskim:mentions | umls-concept:C0205288 | lld:lifeskim |
| pubmed-article:3934284 | pubmed:issue | 1-2 | lld:pubmed |
| pubmed-article:3934284 | pubmed:dateCreated | 1986-1-8 | lld:pubmed |
| pubmed-article:3934284 | pubmed:abstractText | Human natural killer (NK) cells have been reported to express various surface antigens. The majority and the most functionally potent NK cells are Leu-11a (NKP-15) positive cells. Only a small number of functional NK cells express Leu-7 (HNK-1) antigen. In the present study, we have established techniques for immunoelectron microscopic identification of NK cells by mouse monoclonal FITC-conjugated anti-Leu-11a and biotinylated anti-Leu-7 antibodies. Ficoll-Hypaque-isolated peripheral blood lymphocytes (PBL) were reacted with the specific antibodies before or after fixation in a 1% glutaraldehyde/1% paraformaldehyde fixative. Prefixation labeling of viable cells with the antibodies was carried out at 4 degrees C or 37 degrees C. Cells prelabeled with anti-Leu-11a antibody were reacted with secondary antibodies either before or after fixation. Anti-Leu-7 antibody was stained directly via an avidin-biotin-peroxidase (ABC) system, anti-Leu-11a antibody was stained indirectly by the ABC immunoperoxidase procedure via a biotinylated anti-mouse IgG secondary antibody or by a 10 nm or 40 nm colloidal gold-labeled anti-mouse IgG antibody. Results indicate that Leu-7 antigen could be localized by incubation with the specific antibody either before or after 20 min fixation; however, Leu-11a antigen was totally abrogated following the same fixation procedure. The Leu-11a antigen was well stained by the methods of prefixation labeling of cells with anti-Leu-11a antibody and incubation with a biotinylated secondary antibody and the ABC system after fixation. With respect to colloidal gold labeling, better results were obtained when cells were reacted with the gold-labeled antibodies immediately after incubation with anti-Leu-11a antibody but before fixation. Ultrastructurally both Leu-7 positive (+) and Leu-11a positive (+) cells shared common ultrastructural features associated with large granular lymphocytes. Using the above described techniques, we found approximately 2-5% Leu-7+ and 9-15% Leu-11a+ cells in the PBL of healthy donors. The overall results suggest that Leu-11a antigen is more sensitive to glutaraldehyde/paraformaldehyde fixation than Leu-7, since it can be localized only by prefixation labeling procedures; the ABC immunoperoxidase procedure is an ideal technique for labeling NK cells for light and electron microscopic enumeration; the immunogold method provides an adequate technique for labeling NK cells which are designated for ultracytochemical studies. | lld:pubmed |
| pubmed-article:3934284 | pubmed:language | eng | lld:pubmed |
| pubmed-article:3934284 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3934284 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:3934284 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3934284 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3934284 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3934284 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3934284 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3934284 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3934284 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3934284 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3934284 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3934284 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3934284 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3934284 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:3934284 | pubmed:month | Nov | lld:pubmed |
| pubmed-article:3934284 | pubmed:issn | 0022-1759 | lld:pubmed |
| pubmed-article:3934284 | pubmed:author | pubmed-author:KangY HYH | lld:pubmed |
| pubmed-article:3934284 | pubmed:author | pubmed-author:CarlMM | lld:pubmed |
| pubmed-article:3934284 | pubmed:author | pubmed-author:YaffeLL | lld:pubmed |
| pubmed-article:3934284 | pubmed:author | pubmed-author:WatsonL PLP | lld:pubmed |
| pubmed-article:3934284 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:3934284 | pubmed:day | 28 | lld:pubmed |
| pubmed-article:3934284 | pubmed:volume | 84 | lld:pubmed |
| pubmed-article:3934284 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:3934284 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:3934284 | pubmed:pagination | 177-96 | lld:pubmed |
| pubmed-article:3934284 | pubmed:dateRevised | 2004-11-17 | lld:pubmed |
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| pubmed-article:3934284 | pubmed:meshHeading | pubmed-meshheading:3934284-... | lld:pubmed |
| pubmed-article:3934284 | pubmed:year | 1985 | lld:pubmed |
| pubmed-article:3934284 | pubmed:articleTitle | Immunoelectron microscopic identification of human NK cells by FITC-conjugated anti-Leu-11a and biotinylated anti-Leu-7 antibodies. | lld:pubmed |
| pubmed-article:3934284 | pubmed:publicationType | Journal Article | lld:pubmed |
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