Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1985-11-27
pubmed:abstractText
The coding region of reovirus type 3 genomic segment S3, encoding the nonstructural protein sigma NS, was placed under the control of the bacteriophage lambda pL promoter in the Escherichia coli expression plasmid pRC23 (J.C. Lacal, E. Santos, V. Notario, M. Barbacid, S. Yamazaki, H.-F. Kung, C. Seamans, S. McAndrew, and R. Crowl, Proc. Natl. Acad. Sci. USA 81:5305-5309). Derepression of the pL promoter led to the synthesis of a protein of the same molecular weight as sigma NS produced in reovirus-infected L cells. The expressed protein was indistinguishable from authentic sigma NS by peptide mapping with Staphylococcus aureus V8 protease and by immunoblot analysis. Most importantly, the purified protein had nucleic acid-binding properties similar to that previously shown for sigma NS obtained from infected cells. Binding of single-stranded RNAs by recombinant sigma NS protein was inhibited by GTP.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/3932675-1054511, http://linkedlifedata.com/resource/pubmed/commentcorrection/3932675-1266045, http://linkedlifedata.com/resource/pubmed/commentcorrection/3932675-185613, http://linkedlifedata.com/resource/pubmed/commentcorrection/3932675-232223, http://linkedlifedata.com/resource/pubmed/commentcorrection/3932675-320200, http://linkedlifedata.com/resource/pubmed/commentcorrection/3932675-388439, http://linkedlifedata.com/resource/pubmed/commentcorrection/3932675-4509307, http://linkedlifedata.com/resource/pubmed/commentcorrection/3932675-4673272, http://linkedlifedata.com/resource/pubmed/commentcorrection/3932675-4795672, http://linkedlifedata.com/resource/pubmed/commentcorrection/3932675-4818368, http://linkedlifedata.com/resource/pubmed/commentcorrection/3932675-5166483, http://linkedlifedata.com/resource/pubmed/commentcorrection/3932675-5432063, http://linkedlifedata.com/resource/pubmed/commentcorrection/3932675-5723704, http://linkedlifedata.com/resource/pubmed/commentcorrection/3932675-6089191, http://linkedlifedata.com/resource/pubmed/commentcorrection/3932675-6168769, http://linkedlifedata.com/resource/pubmed/commentcorrection/3932675-6300836, http://linkedlifedata.com/resource/pubmed/commentcorrection/3932675-6312421, http://linkedlifedata.com/resource/pubmed/commentcorrection/3932675-664214, http://linkedlifedata.com/resource/pubmed/commentcorrection/3932675-6954490, http://linkedlifedata.com/resource/pubmed/commentcorrection/3932675-7003539, http://linkedlifedata.com/resource/pubmed/commentcorrection/3932675-7029468, http://linkedlifedata.com/resource/pubmed/commentcorrection/3932675-7035855, http://linkedlifedata.com/resource/pubmed/commentcorrection/3932675-7072935, http://linkedlifedata.com/resource/pubmed/commentcorrection/3932675-716218, http://linkedlifedata.com/resource/pubmed/commentcorrection/3932675-7431488, http://linkedlifedata.com/resource/pubmed/commentcorrection/3932675-886647
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0022-538X
pubmed:author
pubmed:issnType
Print
pubmed:volume
56
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
527-33
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1985
pubmed:articleTitle
Synthesis in Escherichia coli of the reovirus nonstructural protein sigma NS.
pubmed:publicationType
Journal Article