Linked Life Data

3931984

Source:http://linkedlifedata.com/resource/pubmed/id/3931984

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1985-12-20
pubmed:abstractText
Survival of Chinese hamster lung (V79) cells, exposed as a function of time to hypothermia in tissue culture, in isosmotic and various hypertonic media was measured using a colony assay. The mechanism of hypothermic cell killing is different above and below 7 degrees C in this cell line. Addition of NaCl or mannitol to increase the tonicity to 400 mOsm greatly decreased the survival at 10 degrees C while addition of KCl had no significant effect. When these experiments were repeated at 5 degrees C, addition of either NaCl, KCl, or mannitol was detrimental to long-term cell survival. Furthermore, addition of mannitol to the medium did not improve survival when cells were stored at 7 degrees C. Addition of KCl at 5 or 10 degrees C or NaCl at 5 degrees C only affected the cells' ability to accumulate sublethal damage, while addition of mannitol at 5 or 10 degrees C affected both of the above and the cold sensitivity of the cells. Addition of NaCl at 10 degrees C only affected the latter. These experiments suggest that prevention of cell swelling by these conditions, while possibly necessary during clinical hypothermic organ storage, is detrimental to single cell survival at these temperatures.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0011-2240
pubmed:author
pubmed:issnType
Print
pubmed:volume
22
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
484-9
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
1985
pubmed:articleTitle
Factors influencing survival of mammalian cells exposed to hypothermia. II. Effects of various hypertonic media.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't