Linked Life Data

3931205

Source:http://linkedlifedata.com/resource/pubmed/id/3931205

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
1985-11-21
pubmed:abstractText
The effect of sucralfate on the proteolytic activity of pepsin towards gastric mucus glycoprotein was investigated. Mucus glycoprotein isolated from pig stomach was incubated with pepsin with and without sucralfate, and the released alpha-amino residues were quantitated. In the absence of sulcralfate the rate of proteolysis was proportional to mucus glycoprotein concentration up to 450 micrograms and remained constant with time of incubation for up to 1 h. The apparent Km value of pepsin towards mucus glycoprotein was 8.7 X 10(-7) M. Introduction of sucralfate to the reaction mixtures resulted in reduction of the rate of glycoprotein proteolysis. The rate of inhibition was proportional to the sucralfate concentration up to 1 microM and was of the competitive type, with an apparent Ki value of 2.0 X 10(-6)M. The results suggest that among the beneficial effects of sucralfate on ulcer healing, one may be attributed to its ability to interfere with peptic digestion of gastric mucus glycoprotein.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0036-5521
pubmed:author
pubmed:issnType
Print
pubmed:volume
20
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
857-60
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1985
pubmed:articleTitle
In vitro inhibition of peptic degradation of porcine gastric mucus glycoprotein by sucralfate.
pubmed:publicationType
Journal Article, In Vitro, Research Support, U.S. Gov't, P.H.S.