Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
19
pubmed:dateCreated
1985-10-31
pubmed:abstractText
To identify a signal involved in transporting a ribosomal protein to the nucleus, we constructed hybrid genes encoding amino-terminal segments of yeast ribosomal protein L3 joined to the amino-terminal end of the entire Escherichia coli beta-galactosidase molecule. The subcellular locations of the corresponding hybrid proteins in yeast were determined by in situ immunofluorescence. The first 21 amino acids of L3 were sufficient to localize beta-galactosidase to the nucleus. This region shows limited homology to portions of other nuclear proteins identified as essential for their transport. Larger fusion proteins were also localized to the nucleus. However, a hybrid protein containing all but the 14 carboxyl-terminal amino acids from L3 initially failed to localize; this defect was corrected by inserting a glycine- and proline-containing bridge between the L3 and beta-galactosidase moieties. The renovated protein was able to associate with ribosomes, suggesting that, in addition to entering the nucleus, this hybrid polypeptide was assembled into 60S ribosomal subunits that were subsequently exported to the cytoplasm.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/3931077-1102604, http://linkedlifedata.com/resource/pubmed/commentcorrection/3931077-1156597, http://linkedlifedata.com/resource/pubmed/commentcorrection/3931077-326416, http://linkedlifedata.com/resource/pubmed/commentcorrection/3931077-3885011, http://linkedlifedata.com/resource/pubmed/commentcorrection/3931077-3897837, http://linkedlifedata.com/resource/pubmed/commentcorrection/3931077-4395100, http://linkedlifedata.com/resource/pubmed/commentcorrection/3931077-46868, http://linkedlifedata.com/resource/pubmed/commentcorrection/3931077-5128352, http://linkedlifedata.com/resource/pubmed/commentcorrection/3931077-5280340, http://linkedlifedata.com/resource/pubmed/commentcorrection/3931077-592361, http://linkedlifedata.com/resource/pubmed/commentcorrection/3931077-6086146, http://linkedlifedata.com/resource/pubmed/commentcorrection/3931077-6088076, http://linkedlifedata.com/resource/pubmed/commentcorrection/3931077-6088992, http://linkedlifedata.com/resource/pubmed/commentcorrection/3931077-6091123, http://linkedlifedata.com/resource/pubmed/commentcorrection/3931077-6096007, http://linkedlifedata.com/resource/pubmed/commentcorrection/3931077-6188533, http://linkedlifedata.com/resource/pubmed/commentcorrection/3931077-6304624, http://linkedlifedata.com/resource/pubmed/commentcorrection/3931077-6305925, http://linkedlifedata.com/resource/pubmed/commentcorrection/3931077-6323016, http://linkedlifedata.com/resource/pubmed/commentcorrection/3931077-6365931, http://linkedlifedata.com/resource/pubmed/commentcorrection/3931077-6383199, http://linkedlifedata.com/resource/pubmed/commentcorrection/3931077-6390341, http://linkedlifedata.com/resource/pubmed/commentcorrection/3931077-6397123, http://linkedlifedata.com/resource/pubmed/commentcorrection/3931077-6501421, http://linkedlifedata.com/resource/pubmed/commentcorrection/3931077-6790520, http://linkedlifedata.com/resource/pubmed/commentcorrection/3931077-6811596, http://linkedlifedata.com/resource/pubmed/commentcorrection/3931077-6814762, http://linkedlifedata.com/resource/pubmed/commentcorrection/3931077-6993285, http://linkedlifedata.com/resource/pubmed/commentcorrection/3931077-7017711, http://linkedlifedata.com/resource/pubmed/commentcorrection/3931077-7035466, http://linkedlifedata.com/resource/pubmed/commentcorrection/3931077-7074038
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:volume
82
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
6561-5
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1985
pubmed:articleTitle
Identification of a nuclear localization signal of a yeast ribosomal protein.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't