Linked Life Data

3930891

Source:http://linkedlifedata.com/resource/pubmed/id/3930891

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1985-11-12
pubmed:abstractText
We have examined the interrelationship between Interleukin 2 (IL 2) and Interferon gamma (IFN gamma) for the induction of cytolytic activity from inactivated cloned cytotoxic T lymphocytes (CTL). In previous work we showed that treatment of cloned CTL with phorbol myristate acetate (PMA) inactivated their cytolytic function and that these inactivated cells provided an experimental approach to define the cytokines necessary for the induction of cytolysis. We further showed that IL 2 could provide a sufficient signal to restore cytolytic activity to inactivated cells by a process that was independent of cellular proliferation. Here we report that reactivation of the lytic response by IL 2 did not correlate with IFN production. IL 2 had the ability to restore lytic activity to PMA-treated cells but did not induce IFN gamma production. Similarly, IL 2 augmented the preexisting lytic response of non-PMA-treated cloned cells without stimulating IFN gamma production. However, PMA-treated and untreated cells had the capacity to produce IFN gamma when cultured with mitogen. These results suggest that IL 2 mediated reactivation of the cytolytic response and IFN gamma production by a cloned CTL appear to be regulated independently.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0277-6766
pubmed:author
pubmed:issnType
Print
pubmed:volume
4
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
299-308
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
1985
pubmed:articleTitle
Phorbol ester-inactivation of cloned cytotoxic T lymphocytes: restoration of lytic activity by interleukin 2 and induction of interferon production are separable events.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't