Linked Life Data

3929083

Source:http://linkedlifedata.com/resource/pubmed/id/3929083

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1985-10-10
pubmed:abstractText
The role of glycoprotein processing in Trypanosoma cruzi association with mammalian host cells (i.e., surface binding and internalization) was studied by using swainsonine - an inhibitor of glycoprotein processing. The presence of swainsonine in co-cultures of blood forms of T. cruzi with either mouse peritoneal macrophages or rat heart myoblasts markedly reduced parasite-host cell association as evidenced by significant decreases in both the percentage of cells associating with the organisms and the number of parasites per cell. This inhibition appeared to result from effects on both the parasite and the host cells since pretreatment of either one reduced its association with the untreated counterpart. The inhibitory effect of swainsonine on the host cells was demonstrable with trypomastigote forms derived from either infected mouse blood or the feces of infected insect vectors, and also with amastigotes. Conversely, treatment of the parasites with swainsonine inhibited their association with the host cells. Studies with blood trypomastigotes revealed that the swainsonine effect was reversible within 3 h. Two observations suggested that swainsonine had induced the parasite to produce defective oligosaccharides rich in mannose. First, swainsonine-treated organisms displayed a greater capacity to bind concanavalin than medium-treated organisms. Second, presence of alpha-methyl mannoside reduced such increased concanavalin A binding. Taken together, the present results suggest important roles for glycoprotein processing and oligosaccharide structures on the surface of both T. cruzi and its host cells in the initial stages of host cell infection.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0166-6851
pubmed:author
pubmed:issnType
Print
pubmed:volume
16
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1-10
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed:year
1985
pubmed:articleTitle
The effect of swainsonine on the association of Trypanosoma cruzi with host cells.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't