Linked Life Data

3928722

Source:http://linkedlifedata.com/resource/pubmed/id/3928722

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
1985-10-22
pubmed:abstractText
As part of an overall evaluation of possible substitutes for the pulpotomy agent formocresol, this study was initiated to compare the antigenicity of the reaction products of protein with formaldehyde, glutaraldehyde, or dimethylsuberimidate (DMS). Rabbits were injected with rabbit serum albumin (RSA) which had been treated with one of the following solutions: phosphate-buffered saline, 2% glutaraldehyde, 4% formaldehyde, or 2% DMS. The antisera from the rabbits were analyzed for elicited antibodies by the enzyme-linked immunosorbent assay (ELISA) and in a horseradish peroxidase (HRP) assay using a spot technique on nitrocellulose paper. These assays demonstrated that DMS-treated RSA was the most antigenic of the reaction products tested. The least provocative was the glutaraldehyde-treated RSA; the reaction product of formaldehyde was intermediate. Our findings suggest that if non-immunogenicity of a pulpotomy agent is a desirable property, then DMS does not meet the criteria of an alternative pulp fixative. In contrast, the relatively low antigenicity of glutaraldehyde reinforces other favorable findings which support its use clinically.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
D
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0022-0345
pubmed:author
pubmed:issnType
Print
pubmed:volume
64
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1225-8
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1985
pubmed:articleTitle
The effect of alternatives to formocresol on antigenicity of proteins.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S.