Linked Life Data

3927524

Source:http://linkedlifedata.com/resource/pubmed/id/3927524

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1985-9-19
pubmed:abstractText
A rapid and improved purification procedure is described for phospholipase A2 from the Indian cobra, Naja naja naja. The procedure is based on affinity chromatography of the venom through Affi-Gel Blue to obtain a 9-fold purification in one step. However, as there are multiple forms of the enzyme in the venom and other proteins do bind to Affi-Gel Blue, further purification is achieved by DE-11 cellulose. Finally, a minor contaminant is removed by Sulfopropyl Sephadex C-25 chromatography. The resulting product was found to be pure by analytical isoelectric focusing and double diffusion precipitin tests. An isoelectric focusing column run with venom collected from a single snake gave a similar profile to that obtained with venom pooled from several snakes.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0041-0101
pubmed:author
pubmed:issnType
Print
pubmed:volume
23
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
457-66
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed:year
1985
pubmed:articleTitle
Affinity chromatography of phospholipase A2 from Naja naja naja (Indian cobra) venom.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S.